Abstract
The folate receptor (FR) is over-expressed on many solid tumors and has been exploited for targeted delivery of folic acid linked liposomes to cancer cells in vitro. In the present study, we developed a novel folic acid (FA) conjugated chitosan (CTS) grafted thiazole orange (TO) complex (FA-CTS-TO), and the formation can be used to label tumor cells. The structure of TO derivatives was confirmed by 1H NMR and MS, and the fluorescence probe of FA-CTS-TO complex was confirmed by Fourier transform infrared analysis and Differential thermal analysis. The in vitro and in vivo of FA-CTS-TO complex were tested in breast cancer cells and the results showed a high targeting specificity in tumor cells with FR over-expressed. Such prominent fluorescence properties demonstrate again that FA-CTS-TO complex as a tumor targeting fluorescence probe is appropriate for breast cancer cells.
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Acknowledgments
This work is financially supported by the National Natural Foundation of China (21072147, 51178289) and the National Science Foundation of Tianjin (NO.09JCYBJC04100, 10JCYBJC10500).
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Fei, XN., Liu, Y. & Li, C. Folate Conjugated Chitosan Grafted Thiazole Orange Derivative with High Targeting for Early Breast Cancer Cells Diagnosis. J Fluoresc 22, 1555–1561 (2012). https://doi.org/10.1007/s10895-012-1094-5
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DOI: https://doi.org/10.1007/s10895-012-1094-5