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Proteomic Analysis of Silkworm Antennae

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Abstract

The silkworm Bombyx mori is an oligophagous insect that feeds mainly on mulberry leaves. The olfactory system of silkworm is a good model to study olfaction in Lepidoptera. Here, we carried out shotgun proteomic analysis and MS sequencing of the silkmoth antennae. A total of 364 proteins were detected, 77 were female specific, 143 were male specific, and 144 were expressed in both male and female antennae. Five odorant-binding proteins, two chemosensory proteins, and one olfactory receptor were identified. They may play a major role in the perception of odorants. An esterase and an aldehyde dehydrogenase were found only in male antennae. Glutathione S-transferases (GSTs) and cytochrome P450s, also found in silkworm antennae, may be involved in the degradation of xenobiotics. Additionally, antioxidation proteins and immunity proteins were identified. Juvenile hormone binding proteins (JHBP), juvenile hormone resistance protein II, and juvenile hormone episode hydrolase (JHEH) were found in the proteomic analysis, which suggests that the antennae are a target for juvenile hormone in the silkworm. Our results provide insight into the expression of proteins in the antennae of silkworm and will facilitate the future functional analysis of silkworm antennae.

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Acknowledgments

This work is funded by the National Basic Research Program of China (2015CB755703), grants from NSFC (31172152 and 31402012) and CAS (KSZD-EW-Z-021-2-1).

Author contributions

X.M. designed the experiment and wrote the manuscript. Y.Z. and H.L. performed experiments and analyzed the data.

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Authors and Affiliations

  1. Key Laboratory of Insect Developmental and Evolutionary Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200032, People’s Republic of China

    Yunpo Zhao, Haichao Li & Xuexia Miao

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  1. Yunpo Zhao
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  2. Haichao Li
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  3. Xuexia Miao
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Correspondence to Xuexia Miao.

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Zhao, Y., Li, H. & Miao, X. Proteomic Analysis of Silkworm Antennae. J Chem Ecol 41, 1037–1042 (2015). https://doi.org/10.1007/s10886-015-0643-1

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  • DOI: https://doi.org/10.1007/s10886-015-0643-1

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