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Grape Seed Proanthocyanidin Extract Attenuates Allergic Inflammation in Murine Models of Asthma

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Abstract

Background

Antioxidants have been suggested to alleviate the pathophysiological features of asthma, and grape seed proanthocyanidin extract (GSPE) has been reported to have powerful antioxidant activity.

Purpose

This study was performed to determine whether GSPE has a therapeutic effect on allergic airway inflammation in both acute and chronic murine model of asthma.

Methods

Acute asthma model was generated by intraperitoneal sensitization of ovalbumin (OVA) with alum followed by aerosolized OVA challenges, whereas chronic asthma model was induced by repeated intranasal challenges of OVA with fungal protease twice a week for 8 weeks. GSPE was administered by either intraperitoneal injection or oral gavage before OVA challenges. Airway hyperresponsiveness (AHR) was measured, and airway inflammation was evaluated by bronchoalveolar lavage (BAL) fluid analysis and histopathological examination of lung tissue. Lung tissue levels of various cytokines, chemokines, and growth factors were analyzed by quantitative polymerase chain reaction and ELISA. Glutathione assay was done to measure oxidative burden in lung tissue.

Results

Compared to untreated asthmatic mice, mice treated with GSPE showed significantly reduced AHR, decreased inflammatory cells in the BAL fluid, reduced lung inflammation, and decreased IL-4, IL-5, IL-13, and eotaxin-1 expression in both acute and chronic asthma models. Moreover, airway subepithelial fibrosis was reduced in the lung tissue of GSPE-treated chronic asthmatic mice compared to untreated asthmatic mice. Reduced to oxidized glutathione (GSH/GSSG) ratio was increased after GSPE treatment in acute asthmatic lung tissue.

Conclusion

GSPE effectively suppressed inflammation in both acute and chronic mouse models of asthma, suggesting a potential role of GSPE as a therapeutic agent for asthma.

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Acknowledgments

This work was supported by a grant (No. 20090086092) from the National Research Foundation of Korea (NRF) to Y.S.C. and a grant (No.2012-302) from Asan Life and Science Institute to Y.S.C.

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Authors and Affiliations

  1. Department of Allergy and Clinical Immunology, Asan Medical Center, University of Ulsan College of Medicine, 88, Olympic-ro 43-gil, Songpa-gu, Seoul, 138-736, Korea

    Taehoon Lee, Hyouk-Soo Kwon, Yoon Su Lee, Tae-Bum Kim, Hee-Bom Moon & You Sook Cho

  2. Asan Institute for Life Science, Seoul, Korea

    Bo-Ram Bang, Mi-Young Park & Keun-Ai Moon

  3. Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine, Suwon, Korea

    Ki-Young Lee

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  1. Taehoon Lee
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Correspondence to You Sook Cho.

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Taehoon Lee and Hyouk-Soo Kwon equally contributed to this article.

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Lee, T., Kwon, HS., Bang, BR. et al. Grape Seed Proanthocyanidin Extract Attenuates Allergic Inflammation in Murine Models of Asthma. J Clin Immunol 32, 1292–1304 (2012). https://doi.org/10.1007/s10875-012-9742-8

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  • DOI: https://doi.org/10.1007/s10875-012-9742-8

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