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Spatio-temporal morphology changes in and quenching effects on the 2D spreading dynamics of cell colonies in both plain and methylcellulose-containing culture media

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Abstract

To deal with complex systems, microscopic and global approaches become of particular interest. Our previous results from the dynamics of large cell colonies indicated that their 2D front roughness dynamics is compatible with the standard Kardar–Parisi–Zhang (KPZ) or the quenched KPZ equations either in plain or methylcellulose (MC)-containing gel culture media, respectively. In both cases, the influence of a non-uniform distribution of the colony constituents was significant. These results encouraged us to investigate the overall dynamics of those systems considering the morphology and size, the duplication rate, and the motility of single cells. For this purpose, colonies with different cell populations (N) exhibiting quasi-circular and quasi-linear growth fronts in plain and MC-containing culture media are investigated. For small N, the average radial front velocity and its change with time depend on MC concentration. MC in the medium interferes with cell mitosis, contributes to the local enlargement of cells, and increases the distribution of spatio-temporal cell density heterogeneities. Colony spreading in MC-containing media proceeds under two main quenching effects, I and II; the former mainly depending on the culture medium composition and structure and the latter caused by the distribution of enlarged local cell domains. For large N, colony spreading occurs at constant velocity. The characteristics of cell motility, assessed by measuring their trajectories and the corresponding velocity field, reflect the effect of enlarged, slow-moving cells and the structure of the medium. Local average cell size distribution and individual cell motility data from plain and MC-containing media are qualitatively consistent with the predictions of both the extended cellular Potts models and the observed transition of the front roughness dynamics from a standard KPZ to a quenched KPZ. In this case, quenching effects I and II cooperate and give rise to the quenched-KPZ equation. Seemingly, these results show a possible way of linking the cellular Potts models and the 2D colony front roughness dynamics.

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Acknowledgments

This work was supported by the Consejo Nacional de Investigaciones Cientficas y Técnicas of Argentina (PIP 2231 and PIP 0602) and the Comisión de Investigaciones Científicas (CIC), Pcia. Bs. As. We acknowledge Silvia Coronato for technical assistance.

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Authors and Affiliations

  1. Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (INIFTA), Universidad Nacional de La Plata (UNLP), CONICET, Sucursal 4, Casilla de Correo 16, 1900, La Plata, Argentina

    N. E. Muzzio, M. A. Pasquale, M. A. C. Huergo, A. E. Bolzán & A. J. Arvia

  2. Cátedra de Patología, Facultad de Ciencias Médicas, UNLP, CIC, Calle 60 y 120, 1900, La Plata, Bs. As., Argentina

    P. H. González

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  1. N. E. Muzzio
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  3. M. A. C. Huergo
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  4. A. E. Bolzán
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Correspondence to M. A. Pasquale.

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Muzzio, N.E., Pasquale, M.A., Huergo, M.A.C. et al. Spatio-temporal morphology changes in and quenching effects on the 2D spreading dynamics of cell colonies in both plain and methylcellulose-containing culture media. J Biol Phys 42, 477–502 (2016). https://doi.org/10.1007/s10867-016-9418-3

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