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Single-Fluorophore Diffusion in a Lipid Membrane over a Subwavelength Aperture

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Abstract

We use submicrometer apertures milled in an aluminium film to study the diffusion dynamics of β-Bodipy-FL-C5-HPC (Bodipy-PC) fluorophores in a lipid dioleoylphosphatidylcholine (DOPC) multilayer. The observation volume is limited by the aperture diameter, well below the optical wavelength. This spatial resolution improvement comes together with an enhancement of the detected fluorescence per molecule as compared to an open sample, with a significant increase up to 3.5 times.

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Authors and Affiliations

  1. Domaine Universitaire de Saint Jérôme, Institut Fresnel, Université Paul Cézanne Aix-Marseille III, CNRS UMR 6133, 13397 Marseille, Cedex 20, France

    Jérôme Wenger, Hervé Rigneault & Pierre-François Lenne

  2. ISIS, Université Louis Pasteur, CNRS UMR 7006, 8 Allée G. Monge, 67000, Strasbourg, France

    José Dintinger

  3. Centre d'Immunologie de Marseille-Luminy, CNRS UMR 6102 INSERM UMR, 631, Parc scientifique de Luminy, Case 906, 13009, Marseille, France

    Didier Marguet

Authors
  1. Jérôme Wenger
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  2. Hervé Rigneault
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  3. José Dintinger
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  4. Didier Marguet
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  5. Pierre-François Lenne
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Correspondence to Pierre-François Lenne.

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Wenger, J., Rigneault, H., Dintinger, J. et al. Single-Fluorophore Diffusion in a Lipid Membrane over a Subwavelength Aperture. J Biol Phys 32, SN1–SN4 (2006). https://doi.org/10.1007/s10867-006-2909-x

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  • DOI: https://doi.org/10.1007/s10867-006-2909-x

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