Abstract
In this study, chitosan–Laponite nanocomposite coatings with bone regenerative potential and controlled drug-release capacity are prepared by electrophoretic deposition technique. The controlled release of a glycopeptide drug, i.e. vancomycin, is attained by the intercalation of the polymer and drug macromolecules into silicate galleries. Fourier-transform infrared spectrometry reveals electrostatic interactions between the charged structure of clay and the amine and hydroxyl groups of chitosan and vancomycin, leading to a complex positively-charged system with high electrophoretic mobility. By applying electric field the charged particles are deposited on the surface of titanium foils and uniform chitosan films containing 25–55 wt% Laponite and 937–1655 µg/cm2 vancomycin are obtained. Nanocomposite films exhibit improved cell attachment with higher cell viability. Alkaline phosphatase assay reveals enhanced cell proliferation due the gradual dissolution of Laponite particles into the culture medium. In-vitro drug-release studies show lower release rate through a longer period for the nanocomposite compared to pristine chitosan.
Graphical Abstract
Similar content being viewed by others
References
Shchipunov Y, Ivanova N, Silant’ev V. Bionanocomposites formed by in situ charged chitosan with clay. Green Chem. 2009;11:1758–61.
Kevadiya BD, Joshi GV, Mody HM, Bajaj HC. Biopolymer–clay hydrogel composites as drug carrier: host–guest intercalation and in vitro release study of lidocaine hydrochloride. Appl Clay Sci. 2011;52:364–7.
Ojijo V, Ray SS. Processing strategies in bionanocomposites. Prog Polym Sci. 2013;38:1543–89.
Pongjanyakul T, Khunawattanakul W, Strachan CJ, Gordond KC, Puttipipatkhachorn S, Rades T. Characterization of chitosan–magnesium aluminum silicate nanocomposite films for buccal delivery of nicotine. Int J Biol Macromol. 2013;55:24–31.
Croisier F, Jerome C. Chitosan-based biomaterials for tissue engineering. Eur Polym J. 2013;49(4):780–92.
Sarasam A, Brown P, Khajotia S, Dmytryk J, Madihally S. Antibacterial activity of chitosan-based matrices on oral pathogens. J Mater Sci. 2008;19(3):1083–90. doi:10.1007/s10856-007-3072-z.
Xie W, Xu P, Wang W, Liu Q. Preparation and antibacterial activity of a water-soluble chitosan derivative. Carbohydr Polym. 2002;50(1):35–40.
Pattnaik S, Nethala S, Tripathi A, Saravanan S, Moorthi A, Selvamurugan N. Chitosan scaffolds containing silicon dioxide and zirconia nano particles for bone tissue engineering. Int J Biol Macromol. 2011;49(5):1167–72.
Cai Q, Gu Z, Fu T, Liu Y, Song H, Li F. Kinetic study of chitosan degradation by an electrochemical process. Polym Bull. 2011;67(4):571–82.
Sinha VR, Singla AK, Wadhawan S, Kaushik R, Kumria R, Bansal K, et al. Chitosan microspheres as a potential carrier for drugs. Int J Pharm. 2004;274(1–2):1–33.
Di Martino A, Sittinger M, Risbud MV. Chitosan: a versatile biopolymer for orthopaedic tissue-engineering. Biomaterials. 2005;26(30):5983–90.
Bernkop-Schnürch A, Dünnhaupt S. Chitosan-based drug delivery systems. Eur J Pharm Biopharm. 2012;81:463–9.
Peter M, Binulal NS, Soumya S, Nair SV, Furuike T, Tamura H, et al. Nanocomposite scaffolds of bioactive glass ceramic nanoparticles disseminated chitosan matrix for tissue engineering applications. Carbohydr Polym. 2010;79(2):284–9.
Khunawattanakul W, Puttipipatkhachorn S, Rades T, Pongjanyakul T. Chitosan–magnesium aluminum silicate nanocomposite films: physicochemical characterization and drug permeability. Int J Pharm. 2010;393:219–29.
Cojocariu A, Profire L, Aflori M, Vasile C. In vitro drug release from chitosan/Cloisite 15A hydrogels. Appl Clay Sci. 2012;57:1–9.
Salcedo I, Aguzzi C, Sandri G, Bonferoni MC, Mori M, Cerezo P, et al. In vitro biocompatibility and mucoadhesion of montmorillonite chitosan nanocomposite: a new drug delivery. Appl Clay Sci. 2012;55:131–7.
Jung H, Kim H-M, Choy YB, Hwang S-J, Choy J-H. Laponite-based nanohybrid for enhanced solubility and controlled release of itraconazole. Int J Pharm. 2008;349:28–290.
Wang Q, Zhang J, Wang A. Alkali activation of halloysite for adsorption and release of ofloxacin. Appl Surf Sci. 2013;287:54–61.
Fan Q, Shan D, Xue H, He Y, Cosnier S. Amperometric phenol biosensor based on laponite clay–chitosan nanocomposite matrix. Biosens Bioelectron. 2007;22:816–21.
Gaharwar AK, Rivera CP, Wu C-J, Schmidt G. Transparent, elastomeric and tough hydrogels from poly(ethylene glycol) and silicate nanoparticles. Acta Biomater. 2011;7:4139–48.
Ghadiri M, Chrzanowski W, Lee WH, Fathi A, Dehghani F, Rohanizadeh R. Physico-chemical, mechanical and cytotoxicity characterizations of Laponite®/alginate nanocomposite. Appl Clay Sci. 2013;58:64–73.
Gaharwar AK, Schexnailder PJ, Kline BP, Schmidt G. Assessment of using Laponite® cross-linked poly(ethylene oxide) for controlled cell adhesion and mineralization. Acta Biomater. 2011;7:568–77.
Shi Q, Li Q, Shan D, Fan Q, Xue H. Biopolymer-clay nanoparticles composite system (Chitosan-laponite) for electrochemical sensing based on glucose oxidase. Mater Sci Eng C. 2008;28:1372–5.
Yang H, Hua S, Wang W, Wang A. Composite hydrogel beads based on chitosan and laponite: preparation, swelling, and drug release behaviour. Iran Polym J. 2011;20(6):479–90.
Esposito S, Leone S. Prosthetic joint infections: microbiology, diagnosis, management and prevention. Int J Antimicrob Agents. 2008;32(4):287–93.
Xie Z, Liu X, Jia W, Zhang C, Huang W, Wang J. Treatment of osteomyelitis and repair of bone defect by degradable bioactive borate glass releasing vancomycin. J Control. Release. 2009;139(2):118–26.
Goodman SB, Yao Z, Keeney M, Yang F. The future of biologic coatings for orthopaedic implants. Biomaterials. 2013;34(13):3174–83.
Corni I, Ryan MP, Boccaccini AR. Electrophoretic deposition: from traditional ceramics to nanotechnology. J Eur Ceram Soc. 2008;28(7):1353–67.
Boccaccini AR, Keim S, Ma R, Li Y, Zhitomirsky I. Electrophoretic deposition of biomaterials. J R Soc Interface. 2010;7(Suppl 5):S581–613.
Gebhardt F, Seuss S, Turhan MC, Hornberger H, Virtanen S, Boccaccini AR. Characterization of electrophoretic chitosan coatings on stainless steel. Mater Lett. 2012;66(1):302–4.
Simchi A, Pishbin F, Boccaccini AR. Electrophoretic deposition of chitosan. Mater Lett. 2009;63(26):2253–6.
Pishbin F, Simchi A, Ryan MP, Boccaccini AR. Electrophoretic deposition of chitosan/45S5 Bioglass® composite coatings for orthopaedic applications. Surf Coat Technol. 2011;205:5260–8.
Ordikhani F, Tamjid E, Simchi A. Characterization and antibacterial performance of electrodeposited chitosan-vancomycin composite coatings for prevention of implant-associated infections. Mater Sci Eng C. 2014;41:240–8.
Ordikhani F, Simchi A. Long-term antibiotic delivery by chitosan-based composite coatings with bone regenerative potential. Appl Surf Sci. 2014;317:56–66.
Ordikhani F, Farani MR, Dehghani M, Tamjid E, Simchi A. Physicochemical and biological properties of electrodeposited graphene oxide/chitosan films with drug-eluting capacity. Carbon. 2015;84:91–102.
Chakraborty SP, Pramanik P, Roy S. A review on emergence of antibioticresistant staphylococcus aureus and role of chitosan nanoparicle in drug delivery. Int J Life Sci Pharma Res. 2012;2:L96–115.
Chakraborty SP, Sahu SK, Pramanik P, Roy S. In vitro antimicrobial activity of nanoconjugated vancomycin against drug resistant Staphylococcus aureus. Int J Pharm. 2012;436(1–2):659–76.
Xu Y, Ren X, Hanna MA. Chitosan/clay nanocomposite film preparation and characterization. J Appl Polym Sci. 2006;99:1684–91.
Besra L, Liu M. A review on fundamentals and applications of electrophoretic deposition (EPD). Prog Mater Sci. 2007;52(1):1–61.
Yuan Q, Shah J, Hein S, Misra RDK. Controlled and extended drug release behavior of chitosan-based nanoparticle carrier. Acta Biomater. 2010;6(3):1140–8.
Ponsonnet L, Reybier K, Jaffrezic N, Comte V, Lagneau C, Lissac M, et al. Relationship between surface properties (roughness, wettability) of titanium and titanium alloys and cell behaviour. Mater Sci Eng C. 2003;23(4):551–60.
Vlacic-Zischke J, Hamlet SM, Friis T, Tonetti MS, Ivanovski S. The influence of surface microroughness and hydrophilicity of titanium on the up-regulation of TGFβ/BMP signalling in osteoblasts. Biomaterials. 2011;32(3):665–71.
Pishbin F, Mourino V, Gilchrist JB, McComb DW, Kreppel S, Salih V, et al. Single-step electrochemical deposition of antimicrobial orthopaedic coatings based on a bioactive glass/chitosan/nano-silver composite system. Acta Biomater. 2013;9(7):7469–79.
Depan D, Kumar AP, Singh RP. Cell proliferation and controlled drug release studies of nanohybrids based on chitosan-g-lactic acid and montmorillonite. Acta Biomater. 2009;5:93–100.
Gaharwar AK, Schexnailder PJ, Jin Q, Wu C-J, Schmidt G. Addition of chitosan to silicate cross-linked PEO for tuning osteoblast cell adhesion and mineralization. Appl Mater Interfaces. 2010;2:3119–27.
Pon-On W, Charoenphandhu N, Teerapornpuntakit J, Thongbunchoo J, Krishnamra N, Tang IM. In vitro study of vancomycin release and osteoblast-like cell growth on structured calcium phosphate-collagen. Mater Sci Eng C. 2013;33(3):1423–31.
Cheng Y, Luo X, Bentley WE, Betz J, Rubloff GW, Buckhout-White S, et al. In situ quantitative visualization and characterization of chitosan electrodeposition with paired sidewall electrodes. Soft Matter. 2010;6:3177–83.
Ghadiri M, Hau H, Chrzanowski W, Agus H, Rohanizadeh R. Laponite clay as a carrier for in situ delivery of tetracycline. RSC Adv. 2013;3:20193–201.
Davies JE, Ajami E, Moineddin R, Mendes VC. The roles of different scale ranges of surface implant topography on the stability of the bone/implant interface. Biomaterials. 2013;34(14):3535–46.
Mieszawska AJ, Fourligas N, Georgakoudi I, Ouhib NM, Belton DJ, Perry CC, et al. Osteoinductive silk-silica composite biomaterials for bone regeneration. Biomaterials. 2010;31(34):8902–10.
Acknowledgments
The authors thank funding support from the Grant Program of Sharif University of Technology (No. G930305) and Elite National Institute (No. ENL 5418).
Author information
Authors and Affiliations
Corresponding author
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Ordikhani, F., Dehghani, M. & Simchi, A. Antibiotic-loaded chitosan–Laponite films for local drug delivery by titanium implants: cell proliferation and drug release studies. J Mater Sci: Mater Med 26, 269 (2015). https://doi.org/10.1007/s10856-015-5606-0
Received:
Accepted:
Published:
DOI: https://doi.org/10.1007/s10856-015-5606-0