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Adsorption characteristics of bovine serum albumin onto alumina with a specific crystalline structure

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Abstract

Bone cement containing alumina particles with a specific crystalline structure exhibits the ability to bond with bone. These particles (AL-P) are mainly composed of delta-type alumina (δ-Al2O3). It is likely that some of the proteins present in the body environment are adsorbed onto the cement and influence the expression of its bioactivity. However, the effect that this adsorption of proteins has on the bone-bonding mechanism of bone cement has not yet been elucidated. In this study, we investigated the characteristics of the adsorption of bovine serum albumin (BSA) onto AL-P and compared them with those of its adsorption onto hydroxyapatite (HA), which also exhibits bone-bonding ability, as well as with those of adsorption onto alpha-type alumina (α-Al2O3), which does not bond with bone. The adsorption characteristics of BSA onto AL-P were very different from those onto α-Al2O3 but quite similar to those onto HA. It is speculated that BSA is adsorbed onto AL-P and HA by interionic interactions, while it is adsorbed onto α-Al2O3 by electrostatic attraction. The results suggest that the specific adsorption of albumin onto implant materials might play a role in the expression of the bone-bonding abilities of the materials.

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References

  1. Hench LL, Splinger RJ, Allen WC, Greenlee TK. Bonding mechanisms at the interface of ceramic prosthetic materials. J Biomed Mater Res. 1972;2:117–41.

    Google Scholar 

  2. Kokubo T, Shigematsu M, Nagashima Y, Tashiro M, Nakamura T, Yamamuro T, Higashi S. Apatite- and wollastonite-containing glass–ceramics for prosthetic application. Bull Inst Chem Res Kyoto Univ. 1982;60:260–8.

    Google Scholar 

  3. Kokubo T, Kushitani H, Sakka S, Kitsugi T, Yamamuro T. Solutions able to reproduce in vivo surface–structure changes in bioactive glass–ceramic A-W. J Biomed Mater Res. 1990;24:721–34.

    Article  Google Scholar 

  4. Kokubo T, Takadama H. How useful is SBF in predicting in vivo bone bioactivity? Biomaterials. 2006;27:2907–15.

    Article  Google Scholar 

  5. Ohtsuki C, Aoki Y, Kokubo T, Fujita Y, Kotani S, Yamamuro T. Bioactivity of limestone and abalone shell. In: Transactions of the 11th annual meeting of Japanese society for biomaterials. 1989. p. 12.

  6. Fujita Y, Yamamuro T, Nakamura T, Kotani S, Kokubo T, Ohtsuki C. The bonding behavior of limestone and abalone shell to bone. In: Transactions of the 11th annual meeting of Japanese society for biomaterials. 1989. p. 3.

  7. Ohtsuki C, Kokubo T, Neo M, Kotani S, Yamamuro T, Nakamura T, Bando Y. Bone-bonding mechanism of sintered beta-3CaO·P2O5. Phosphorus Res Bull. 1991;1:191–6.

    Google Scholar 

  8. Kotani S, Fujita Y, Kitsugi T, Nakamura T, Yamamuro T, Ohtsuki C, Kokubo T. Bone bonding mechanism of β-tricalcium phosphate. J Biomed Mater Res. 1991;25:1303–15.

    Article  Google Scholar 

  9. Kobayashi M, Kikutani T, Kokubo T, Nakamura T. Direct bone formation on alumina bead composite. J Biomed Mater Res. 1997;37:555–65.

    Google Scholar 

  10. Okada K, Kobayashi M, Neo M, Shinzato S, Matsushita M, Kokubo T, Nakamura T. Ultrastructure of the interface between alumina bead composite and bone. J Biomed Mater Res. 2000;49:106–11.

    Article  Google Scholar 

  11. Shinzato S, Kobayashi M, Choju K, Kokubo T, Nakamura T. Bone-bonding behavior of alumina bead composite. J Biomed Mater Res. 1999;49:287–300.

    Article  Google Scholar 

  12. Shinzato S, Nakamura T, Kokubo T, Kitamura Y. Composites consisting of poly(methyl methacrylate) and alumina powder: an evaluation of their mechanical and biological properties. J Biomed Mater Res. 2002;60:585–91.

    Article  Google Scholar 

  13. Nishio K, Neo M, Akiyama H, Okada Y, Kokubo T, Nakamura T. Effects of apatite and wollastonite containing glass–ceramic powder and two types of alumina powder in composites on osteoblastic differentiation of bone marrow cells. J Biomed Mater Res. 2001;55:164–76.

    Article  Google Scholar 

  14. Puleo DA, Nanci A. Understanding and controlling the bone-implant interface. Biomater. 1999;20:2311–21.

    Article  Google Scholar 

  15. Nakamura M, Sekijima Y, Nakamura S, Kobayashi T, Niwa K, Yamashita K. Role of blood coagulation components as intermediators of high osteoconductivity of electrically polarized hydroxyapatite. J Biomed Mater Res A. 2006;79:627–34.

    Article  Google Scholar 

  16. Castner DG, Ratner BD. Biomedical surface science: foundations to frontiers. Surf Sci. 2002;500:28–60.

    Article  Google Scholar 

  17. Tirrell M, Kokkoli E, Biesalski M. The role of surface science in bioengineered materials. Surf Sci. 2002;500:61–83.

    Article  Google Scholar 

  18. Curry S, Brick P, Franks NP. Fatty acid binding to human serum albumin: new insights from crystallographic studies. Biochim Biophys Acta. 1999;1441:131–40.

    Article  Google Scholar 

  19. Pitt WG, Park K, Cooper SL. Sequential protein adsorption and thrombus deposition on polymeric biomaterials. J Colloid Interf Sci. 1986;111:343–62.

    Article  Google Scholar 

  20. Hayashi J, Kawashita M, Miyazaki T, Kamitakahara M, Ioku K, Kanetaka H. Comparison of adsorption behavior of bovine serum albumin and osteopontin on hydroxyapatite and alumina. Phosphorus Res Bull. 2012;26:23–8.

    Article  Google Scholar 

  21. Hayashi J, Kawashita M, Miyazaki T, Kudo T, Kanetaka H, Hashimoto M. MC3T3-E1 cell response to hydroxyapatite and alpha-type alumina adsorbed with bovine serum albumin. Key Eng Mater. 2013;529–530:365–9.

    Google Scholar 

  22. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein–dye binding. Anal Biochem. 1976;72:248–54.

    Article  Google Scholar 

  23. Kawashita M, Kamitani A, Miyazaki T, Matsui N, Li Z, Kanetaka H, Hashimoto M. Zeta potential of alumina powders with different crystalline phases in simulated body fluid. Mater Sci Eng C. 2012;32:2617–22.

    Article  Google Scholar 

  24. Kawasaki T, Takahashi S, Ikeda K. Hydroxyapatite high-performance liquid chromatography: column performance for proteins. Eur J Biochem. 1985;152:361–71.

    Article  Google Scholar 

  25. Kawasaki T, Ikeda K, Takahashi S, Kuboki Y. Further study of hydroxyapatite high-performance liquid chromatography using both proteins and nucleic acids, and a new technique to increase chromatographic efficiency. Eur J Biochem. 1986;155:249–57.

    Article  Google Scholar 

  26. Kawashita M, Hayashi J, Kudo T, Kanetaka H, Li Z, Miyazaki T, Hashimoto M. MC3T3-E1 and RAW264.7 cell response to hydroxyapatite and alpha-type alumina adsorbed with bovine serum albumin. J Biomed Mater Res Part A. doi:10.1002/jbm.a.34861.

  27. Hirayama K, Akashi S, Furuya M, Fukuhara K. Rapid confirmation and revision of the primary structure of bovine serum albumin by ESIMS and FRIT-FAB LC/MS. Biochem Biophys Res Commun. 1990;173:639–46.

    Article  Google Scholar 

  28. Metivier R, Leray I, Lefevre JP, Auberger MR, Szydliwski NZ, Valeur B. Characterization of alumina surfaces by fluorescence spectroscopy. Phys Chem. 2003;5:758–66.

    Google Scholar 

  29. Wischert R, Laurent P, Coperet C, Delbecq F, Sautet P. γ-Alumina: the essential and unexpected role of water for the structure, stability, and reactivity of “defect” sites. J Am Chem Soc. 2012;134:14430–49.

    Article  Google Scholar 

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Acknowledgments

This work was partially supported by a research grant from the Inamori Foundation, Japan and a Grant-in-Aid for Scientific Research from The Ministry of Education, Culture, Sports, Science and Technology, Japan. The authors thank Mr. Okano and Mr. Kaji, Taihei Chemical Industrial Co. Ltd., for providing the HA powder. They also thank Prof. Ishida, Tohoku University, and Dr. Maeda, Nagoya Institute of Technology, for the use of the instrument to measure the specific surface areas.

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Authors and Affiliations

  1. Graduate School of Biomedical Engineering, Tohoku University, Sendai, 980-8579, Japan

    Masakazu Kawashita & Junpei Hayashi

  2. College of Chemistry and Chemical Engineering, Guangxi University, Nanning, 530004, China

    Zhixia Li

  3. Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology, Kitakyushu, 808-0196, Japan

    Toshiki Miyazaki

  4. Japan Fine Ceramics Center, Nagoya, 456-8587, Japan

    Masami Hashimoto

  5. Graduate School of Dentistry, Tohoku University, Sendai, 980-8575, Japan

    Hiroki Hihara & Hiroyasu Kanetaka

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  1. Masakazu Kawashita
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  2. Junpei Hayashi
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  3. Zhixia Li
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  4. Toshiki Miyazaki
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  5. Masami Hashimoto
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  7. Hiroyasu Kanetaka
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Correspondence to Masakazu Kawashita.

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Kawashita, M., Hayashi, J., Li, Z. et al. Adsorption characteristics of bovine serum albumin onto alumina with a specific crystalline structure. J Mater Sci: Mater Med 25, 453–459 (2014). https://doi.org/10.1007/s10856-013-5086-z

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  • DOI: https://doi.org/10.1007/s10856-013-5086-z

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