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Novel macro-microporous gelatin scaffold fabricated by particulate leaching for soft tissue reconstruction with adipose-derived stem cells

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Abstract

The restoration of body contours as shaped by adipose tissue remains a clinical challenge specifically in patients who have experienced loss of contour due to trauma, surgical removal of tumours or congenital abnormalities. We have developed a novel macro-microporous biomaterial for use in soft tissue re-bulking and augmentation. Alginate beads provided the pore template for the construct. Incorporation, and subsequent dissolution, of the beads within a 7 % (w/v) gelatin matrix, produced a highly porous scaffold with an average pore size of 2.01 ± 0.08 mm. The ability of this scaffold to support the in vitro growth and differentiation of human adipose-derived stem cells (ADSCs) was then investigated. Histological analysis confirmed that the scaffold itself provided a suitable environment to support the growth of ADSCs on the scaffold walls. When delivered into the macropores in a fibrin hydrogel, ADSCs proliferated and filled the pores. In addition, ADSCs could readily be differentiated along the adipogenic lineage. These results therefore describe a novel scaffold that can support the proliferation and delivery of ADSCs. The scaffold is the first stage in developing a clinical alternative to current treatment methods for soft tissue reconstruction.

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Acknowledgments

This work was supported by charitable donations to the Blond McIndoe Research Foundation.

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Authors and Affiliations

  1. Brighton Sussex Medical School, Brighton, UK

    Manraj K. Phull & Diana J. Lawrence-Watt

  2. Blond McIndoe Research Foundation, Queen Victoria Hospital, Holtye Road, East Grinstead, West Sussex, RH19 3DZ, UK

    Trevor Eydmann, Judy Roxburgh, Justin R. Sharpe, Diana J. Lawrence-Watt & Yella Martin

  3. School of Pharmacy and Biomolecular Sciences, University of Brighton, Brighton, UK

    Gary Phillips & Yella Martin

Authors
  1. Manraj K. Phull
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  2. Trevor Eydmann
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  3. Judy Roxburgh
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  4. Justin R. Sharpe
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  5. Diana J. Lawrence-Watt
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  6. Gary Phillips
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  7. Yella Martin
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Correspondence to Yella Martin.

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Phull, M.K., Eydmann, T., Roxburgh, J. et al. Novel macro-microporous gelatin scaffold fabricated by particulate leaching for soft tissue reconstruction with adipose-derived stem cells. J Mater Sci: Mater Med 24, 461–467 (2013). https://doi.org/10.1007/s10856-012-4806-0

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  • DOI: https://doi.org/10.1007/s10856-012-4806-0

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