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PAN hollow fiber membranes elicit functional hippocampal neuronal network

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Abstract

This study focuses on the development of an advanced in vitro biohybrid culture model system based on the use of hollow fibre membranes (HFMs) and hippocampal neurons in order to promote the formation of a high density neuronal network. Polyacrylonitrile (PAN) and modified polyetheretherketone (PEEK-WC) membranes were prepared in hollow fibre configuration. The morphological and metabolic behaviour of hippocampal neurons cultured on PAN HF membranes were compared with those cultured on PEEK-WC HF. The differences of cell behaviour between HFMs were evidenced by the morphometric analysis in terms of axon length and also by the investigation of metabolic activity in terms of neurotrophin secretion. These findings suggested that PAN HFMs induced the in vitro reconstruction of very highly functional and complex neuronal networks. Thus, these biomaterials could potentially be used for the in vitro realization of a functional hippocampal tissue analogue for the study of neurobiological functions and/or neurodegenerative diseases.

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Authors and Affiliations

  1. Institute on Membrane Technology, National Research Council of Italy, ITM-CNR, c/o University of Calabria, Via P. Bucci, cubo 17/C, 87030, Rende, CS, Italy

    Sabrina Morelli, Antonella Piscioneri, Simona Salerno, Franco Tasselli, Enrico Drioli & Loredana De Bartolo

  2. Department of Chemical Engineering and Materials of University of Calabria, Via P. Bucci cubo 45/A, 87030, Rende, CS, Italy

    Enrico Drioli

  3. Comparative Neuroanatomy Laboratory, Department of Ecology, University of Calabria, Via P. Bucci 4/B, 87030, Rende, CS, Italy

    Anna Di Vito, Giuseppina Giusi & Marcello Canonaco

  4. WCU Energy Engineering Department, Hanyang University, Seoul, South Korea

    Enrico Drioli

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  1. Sabrina Morelli
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  2. Antonella Piscioneri
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  3. Simona Salerno
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  4. Franco Tasselli
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  6. Giuseppina Giusi
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  7. Marcello Canonaco
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Correspondence to Sabrina Morelli.

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Morelli, S., Piscioneri, A., Salerno, S. et al. PAN hollow fiber membranes elicit functional hippocampal neuronal network. J Mater Sci: Mater Med 23, 149–156 (2012). https://doi.org/10.1007/s10856-011-4484-3

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  • DOI: https://doi.org/10.1007/s10856-011-4484-3

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