Abstract
The purpose of the present study was to determine in vitro the effects of different surface topographies and chemistries of commercially pure titanium (cpTi) and diamond-like carbon (DLC) surfaces on osteoblast growth and attachment. Microgrooves (widths of 2, 4, 8 and 10 μm and a depth of 1.5–2 μm) were patterned onto silicon (Si) substrates using microlithography and reactive ion etching. The Si substrates were subsequently vapor coated with either cpTi or DLC coatings. All surfaces were characterized using atomic force microscopy (AFM), scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS) and contact angle measurements. Using the MG63 Osteoblast-Like cell line, we determined cell viability, adhesion, and morphology on different substrates over a 3 day culture period. The results showed cpTi surfaces to be significantly more hydrophilic than DLC for groove sizes larger than 2 μm. Cell contact guidance was observed for all grooved samples in comparison to the unpatterned controls. The cell viability tests indicated a significantly greater cell number for 8 and 10 μm grooves on cpTi surfaces compared to other groove sizes. The cell adhesion study showed that the smaller groove sizes, as well as the unpatterned control groups, displayed better cell adhesion to the substrate.
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K. ANSELME, Biomat 21 (2000) 667
B. D. BOYAN, T. W. HUMMERT, D. D. DEAN and Z. SCHWARTZ, Biomat 17 (1996) 137
J. C. KELER, C. M. STANFORD, J. P. WIGHTMAN, R. A. DRAUGHN and R. ZAHARIAS, J. Biomed. Mater. Res. 28 (1994) 939
U. MEYER, D. H. SZULCZEWSKI, K. MOLLER, H. HEIDE and D. B. JONES, Cells and Mater. 3 (1993) 129
D. DE SANTIS, C. GUERRIERO, P. F. NOCINI, A. UNGERSBOCK, G. RICHARDS, P. GOTTE and U. ARMATO, J. Mater. Sci. Mater in Med. 7 (1996) 21
N. ABDESSAMAD and M. F. HATMAND J. Biomed. Mater. Res. 24 (1990) 861
C. R. HOWLETT, M. D. M. EVANS, W. R. WALSH, J. GRAHAM and J. G. STEELE, Biomat. 15 (1994) 213
D. A. PULEO and R. BIZIOS J. Biomed. Mater. Res. 26 (1992) 291
A. HUNTER, C. W. ARCHER, P. S. WALKER and G. W. BLUNN, Biomat. 16 (1995) 287
P. CLARK, G. A. DUNN, A. KNIBBS and M. PECKHAM, Int. J. Biochem. Cell Biol,. 34 (2002) 816
A. I. TEIXEIRA, G. A. ABRAMS, C. J. MURPHY and P. F. NEALEY J. Vac. Sci. & Technol. B 21 (2003) 683
S. LENHERT, M. B. MEIER, U. MEYER, L. CHI and H. P. WIESMANN Biomat. 26 (2005) 563
s. g. steinemann , Periodont. 2000, 17 (1998) 7
S. SZMUKLER-MONCLER, D. PERRIN, V. AHOSSI, G. MAGNIN and J. P. BERNARD, J. Biomed. Mater. Res. 68B (2004) 149
D. L. COCHRAN, J. SIMPSON, H. WEBER and D. BUSER, Int. J. Oral Maxillo. Imp. 9 (1994) 289
K. T. BOWERS, J. C. KELLER, B. A. RANDOLPH, D. G. WICK and C. M. MICHAELS, Int. J. Oral Maxillo. Imp. 7 (1992) 302
C. M. MICHAELS, J. C. KELLER, C. M. STANFORD and M. SOLURSH, J. Dent. Res. 68 (1989) 276
J. Y. MARTIN, Z. SCHWARTZ, W. HUMMERT, D. M. SCHRAUB, J. SIMPSON, J. LANKFORD JR, D. D. DEAN, D. L. COCHRAN and B. D. BOYAN, J. Biomed. Mater. Res. 29 (1995) 389
K. KIESWETTER, Z. SCHWARTZ, T. W. HUMMERT, D. L. COCHRAN, J. SIMPSON, D. D. DEAN and B. D. BOYAN, J. Biomed. Mater. Res. A 32 (1996) 55
J. LINCKS, B. D. BOYAN, C. R. BLANCHARD, C. H. LOHMANN, Y. LIU, D. L. COCHRAN, D. D. DEAN and Z. SCHWARTZ, Biomat. 19 (1998) 2219
D. D. DELIGIANNI, N. KATSALA, S. LADAS, D. SOTIROPOULOU, J. AMEDEE and Y. F. MISSIRLIS, Biomat. 22 (2001) 1241
M. BIGERELLE, K. ANSELME, B. NOËL, I. RUDERMAN, P. HARDOUIN and A. IOST, Biomat. 23 (2002) 1563
P. DUCHEYNE, G. WILLEMS, M. MARTENS and J. HELSEN, J. Biomed. Mater. Res. 18 (1984) 293
J. L. WOODMAN, J. J. JACOBS, J. O. GALANTE and R. M. URBAN, J. Ortho. Res. 1 (1984)421
S. AISENBERG and R. CHABOT, J. App. Phys. 42 (1971) 2953
H. C. TSAI and D. B. BOGY, J. Vac. Sci. Technol. A5 (1987) 3287
F. JANSEN, M. MACHONKIN, S. KAPLAN and S. HARK, J. Vac. Sci. Technol. A3 (1985) 605
J. C. ANGUS and F. JANSEN, J. Vac. Sci. & Technol. A6 (1988) 1778
D. R. MCKENZIE, R. C. MCPHEDRAN, N. SAVVIDES and L. C. BOTTER, Philos. Mag. 48 (1983) 341
C. V. DESHPANDEY and R. F. BUNSHAH, J. Vac. Sci. & Technol. A7 (1989) 2294
A. MATTHEWS and S. S. ESKILDEN, Diam. Films. 13 (1993) 1
A. ERDEMIR, M. SWITALA, R. WEI and P. WILBUR, Surf. Coat. Technol. 50 (1991) 17
M. ALLEN, F. LAW and N. RUSHTON, Clin. Mater. 17 (1994) 1
J. JANG , J. H. MOON, E. J. HAN and S. J. CHUNG, Thin Solid Films, 341 (1999)
M. W. PHANEUF, Micron. 30 (1999) 277
M. WIELAND, B. CHEHROUDI, M. TEXTOR and D. M. BRUNETTE, J. Biomed. Mater. Res. 60 (2002) 434
T. K. OLSON , R. G. LEE and J. C. MORGAN, in “18th International Symposium for Testing and Failure Analysis (ISTFA 92)” (Materials Park, Ohio: ASM International, 1992)
b . l. GABRIEL, SEM: A User's Manual For Materials Science (Metals Park, Ohio 44073: American Society for Metals, 1985)
General Electric Company G.E. in http://www.gewater.com/library/tp/772_Hydrophilicity_and.jsp, 1997–2005
S. A. REDEY, S. RAZZOUK, C. REY, D. BERNACHE-ASSOLLANT, G. LEROY, M. NARDIN and G. COURNOT, J. Biomed. Mater. Res. 45 (1999) 140
T. G. VAN KOOTEN, J. M. SCHAKENRAAD, H. C. VAN DER MEI and H. J. BUSSCHER, Biomat. 13 (1992) 897
K. MATSUZAKA, X. F. WALBOOMERS, M. YOSHINARI, T. INOUE and J. A. JANSEN, Biomat. 24 (2003) 2711
E. EISENBARTH, P. LINEZ, V. BIEHL, D. VELTEN, J. BREME and H. F. HILDEBRAND, Biomol. Eng. 19 (2002) 233
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The authors would like to warmly thank Dr Z. Xie (Materials Science, University of New South Wales) for assistance with FIB, and Mr. Tony Romeo and Dr Ian Kaplin (Electron Microscopy Unit, University of Sydney) for assistance with electron microscopy.
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Ismail, F.S.M., Rohanizadeh, R., Atwa, S. et al. The influence of surface chemistry and topography on the contact guidance of MG63 osteoblast cells. J Mater Sci: Mater Med 18, 705–714 (2007). https://doi.org/10.1007/s10856-006-0012-2
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DOI: https://doi.org/10.1007/s10856-006-0012-2