Abstract
Interaction of single-cell protein of Spirulina platensis with aqueous AgNO3 and HAuCl4 was investigated for the synthesis of Ag, Au and Au core—Ag shell nanoparticles. Biological reduction and extracellular synthesis of nanoparticles were achieved in 120 h at 37 °C at pH 5.6. The nanometallic dispersions were characterized by surface plasmon absorbance measuring at 424 and 530 nm for Ag and Au nanoparticles, respectively. For bimetallic nanoparticles, absorption peak was observed at 509, 486 and 464 nm at 75:25, 50:50 and 25:75 (Au:Ag) mol concentrations, respectively. High-resolution transmission electron microscopy showed formation of nanoparticles in the range of 7–16 (silver), 6–10 (gold) and 17–25 nm (bimetallic 50:50 ratio). XRD analysis of the silver and gold nanoparticles confirmed the formation of metallic silver and gold. Fourier transform infrared spectroscopic measurements revealed the fact that the protein is the possible biomolecule responsible for the reduction and capping of the biosynthesized nanoparticles.
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Brust M, Kiely CJ (2002) Colloids Surf A Physicochem Eng Asp 202:175. doi:https://doi.org/10.1016/S0927-7757(01)01087-1
Kowshik M, Ashtaputre S, Kharrazi S, Vogel W, Urban J, Kulkarani SK et al (2003) Nanotechnology 14:95. doi:https://doi.org/10.1088/0957-4484/14/1/321
Huang H, Yang X (2005) Colloids Surf A Physicochem Eng Asp 255:11. doi:https://doi.org/10.1016/j.colsurfa.2004.12.020
Mandal S, Phadtare S, Sastry M (2005) Curr Appl Phys 5:118. doi:https://doi.org/10.1016/j.cap.2004.06.006
Wang C, Flynn NT, Langer R (2004) Adv Mater 16:1074. doi:https://doi.org/10.1002/adma.200306516
Nicewarner-Pena SR, Freeman RG, Reiss BD, He L, Pena J, Walton ID et al (2001) Science 294:137. doi:https://doi.org/10.1126/science.294.5540.137
Han M, Gao X, Su JZ, Nie S (2001) Nat Biotechnol 19:631. doi:https://doi.org/10.1038/90228
Joshi HM, Bhumkar DR, Kalpana J, Varsha P, Murali S (2006) Langmuir 22:300. doi:https://doi.org/10.1021/la051982u
Zhilong Shi, Neoh KG, Kang ET (2004) Langmuir 20:6847. doi:https://doi.org/10.1021/la049132m
Elechiguerra JL, Burt JL, Morones RJ, Camacho A, Gao X, Lara HH et al (2005) Nanobiotechnol 3:1. doi:https://doi.org/10.1186/1477-3155-3-1
Taton TA, Mirkin CA, Letsinger RL (2000) Science 289:1757. doi:https://doi.org/10.1126/science.289.5485.1757
Cao YC, Jin R, Mirkin CA (2002) Science 297:1536. doi:https://doi.org/10.1126/science.297.5586.1536
Sandhu KK, McIntosh CM, Simard JM, Smith SW, Rotello VM (2002) Bioconjugate Chem B 13:3. doi:https://doi.org/10.1021/bc015545c
Gericke M, Pinches A (2006) Hydrometallurgy 83:132. doi:https://doi.org/10.1016/j.hydromet.2006.03.019
Mandal D, Bolander ME, Mukhopadhyay C, Sarkar G, Mukherjee P (2006) Appl Microbiol Biotechnol 69:485. doi:https://doi.org/10.1007/s00253-005-0179-3
Mann S (1993) Nature 365:499. doi:https://doi.org/10.1038/365499a0
Oliver S, Kuperman A, Coombs N, Lough A, Ozin GA (1995) Nature 378:47. doi:https://doi.org/10.1038/378047a0
Kroger N, Deutzmann R, Sumper M (1999) Science 286:1129. doi:https://doi.org/10.1126/science.286.5442.1129
Shankar SS, Rai A, Ankamwar B, Singh A, Ahmad A, Sastry M (2004) Nat Mater 3:482. doi:https://doi.org/10.1038/nmat1152
Shankar SS, Ahmad A, Pasricha R, Sastry M (2003) J Mater Chem 13:1822. doi:https://doi.org/10.1039/b303808b
Shiv SS, Rai A, Ahmad A, Sastry M (2004) J Colloid Interface Sci 275:496. doi:https://doi.org/10.1016/j.jcis.2004.03.003
Shiv SS, Ahmed A, Sastry M (2003) Biotechnol Prog 19:1627. doi:https://doi.org/10.1021/bp034070w
Prathap CS, Chaudhary M, Pasricha R, Ahmad A, Sastry M (2006) Biotechnol Prog 22:577. doi:https://doi.org/10.1021/bp0501423
Huang J, Li Q, Sun D, Lu Y, Su Y, Yang X et al (2007) Nanotechnology 18:105104. doi:https://doi.org/10.1088/0957-4484/18/10/105104
Scarano G, Morelli E (2003) Plant Sci 165:803. doi:https://doi.org/10.1016/S0168-9452(03)00274-7
Konishi Y, Nomura T, Tsukiyama T, Saitoh N (2004) Trans Mater Res Soc Jpn 29:2341
Singaravelu G, Arockyamary JS, Ganesh Kumar V, Govindaraju K (2007) Colloids Surf B Biointerf 57:97. doi:https://doi.org/10.1016/j.colsurfb.2007.01.010
Gadd GM (1990) Experientia 46:834. doi:https://doi.org/10.1007/BF01935534
Kuyucak N, Volesky B, Raton FL (1990) Biosorption of heavy metals. CRC Press, Boca Raton, p 173
Bender J, Gould JP, Vatcharapijiarn Y, Young JS, Phillip S (1994) Water Environ Res 66:679
Hameed A, Hasnain S (2005) Chin J Oceanol Limnol 23:433. doi:https://doi.org/10.1007/BF02842688
Gardea-Torresdey JL, Becker-Hapak KM, Hosea JM, Darnell DW (1990) Environ Sci Technol 19:1372. doi:https://doi.org/10.1021/es00079a011
Kaplan D, Christiaen D, Arad SM (1987) Appl Environ Microbiol 53:2953
Zhang W, Majidi V (1994) Environ Sci Technol 28:1577. doi:https://doi.org/10.1021/es00058a007
Ayehunie S, Belay A, Baba T, Ruprecht R (1998) J Acq Imm Differ Syn 18:7
Mulvaney P (1996) Langmuir 12:788. doi:https://doi.org/10.1021/la9502711
Caruso F, Furlong DN, Ariga K, Ichinose I, Kunitake T (1998) Langmuir 14:4559. doi:https://doi.org/10.1021/la971288h
Van de Weert M, Haris PI, Hennink WE, Crommelin DJA (2001) Anal Biochem 297:160. doi:https://doi.org/10.1006/abio.2001.5337
Mohamed ZA (2001) Water Res 35:4405. doi:https://doi.org/10.1016/S0043-1354(01)00160-9
Philippis RD, Sili C, Paperi R, Vincenzini M (2001) J Appl Phycol 13:293. doi:https://doi.org/10.1023/A:1017590425924
Gardea-Torresdey JL, Aarenas JI, Webb R, Fransisco NMC, Tieman KJ (1997) J Hazard Subst Res 3:1
Gole A, Dash CV, Ramachandran V, Mandale AB, Sainkar SR, Rao M et al (2001) Langmuir 17:1674. doi:https://doi.org/10.1021/la001164w
Selvakannan PR, Mandal S, Phadtare S, Renu Pasricha, Sastry M (2003) Langmuir 19:3545. doi:https://doi.org/10.1021/la026906v
Nair B, Pradeep T (2002) Cryst Growth Des 2:293. doi:https://doi.org/10.1021/cg0255164
Senapati S, Ahmad A, Khan MI, Sastry M, Kumar R (2005) Small 1:517. doi:https://doi.org/10.1002/smll.200400053
Hu Y, Li C, Gu F, Zhao Y (2007) J Alloy Comp 432:L5. doi:https://doi.org/10.1016/j.jallcom.2006.05.134
Han SW, Kim Y, Kim K (1998) J Colloid Interface Sci 208:272. doi:https://doi.org/10.1006/jcis.1998.5812
Macdonald IDG, Smith WE (1996) Langmuir 12:706. doi:https://doi.org/10.1021/la950256w
Keating CD, Kovaleski KK, Natan MJ (1998) J Phys Chem B 102:9414. doi:https://doi.org/10.1021/jp982724r
Kumar CV, McLendon GL (1997) Chem Mater 9:863. doi:https://doi.org/10.1021/cm960634y
Gole A, Dash C, Sainkar SR, Mandale AB, Rao M, Sastry M (2000) Anal Chem 72:1401. doi:https://doi.org/10.1021/ac000099s
Ahmed A, Mukherjee P, Senapati S, Mandal D, Islam Khan M, Kumar R et al (2003) Colloids Surf B 28:313. doi:https://doi.org/10.1016/S0927-7765(02)00174-1
Panigrahi S, Kundu S, Ghosh SK, Sudip Nath, Pal T (2005) Colloids Surf A 264:133. doi:https://doi.org/10.1016/j.colsurfa.2005.04.017
Wang S, Shi G (2007) Mater Chem Phys 102:255. doi:https://doi.org/10.1016/j.matchemphys.2006.12.014
Schmid G (1994) Clusters and colloids. VCH, Weinheim
Toshima N, Yonezawa (1998) J Chem 11:1179
Malin MP, Murphy CJ (2002) Nano Lett 2:1235. doi:https://doi.org/10.1021/nl025774n
Ah CS, Hong SD, Jang DJ (2001) J Phys Chem B 105:7871. doi:https://doi.org/10.1021/jp0113578
Mallik K, Mandal M, Pradhan N, Pal T (2001) Nano Lett 1:319. doi:https://doi.org/10.1021/nl0100264
Cao YW, Jin R, Mirkin CA (2001) J Am Chem Soc 123:7961. doi:https://doi.org/10.1021/ja011342n
Caruso F (2001) Adv Mater 13:11. doi :10.1002/1521-4095(200101)13:1≤11::AID-ADMA11≥3.0.CO;2-N
Schmid G (1992) Chem Rev 92:1709. doi:https://doi.org/10.1021/cr00016a002
III Aiken JD, Finke RG (1999) J Mol Catal A 145:1. doi:https://doi.org/10.1016/S1381-1169(99)00098-9
Henglein (1993) J Phys Chem 97:457
Srnova-Sloufova I, Vickova B, Bastl Z, Hasslett TL (2004) Langmuir 20:3407. doi:https://doi.org/10.1021/la0302605
Bohren CF, Huffman DR (1983) Absorption and scattering of light by small particles. Wiley, New York
Rai A, Chaudhary M, Ahmed A, Bhargava S, Sastry M (2007) Mater Res Bull 42:1212. doi:https://doi.org/10.1016/j.materresbull.2006.10.019
Acknowledgements
G.S. and K.G. thank the Department of Science and Technology (DST), New Delhi, Government of India, for financial assistance. The HR-TEM assistance of SAIF, IIT, Chennai, is gratefully acknowledged. The authors thank Prof. L. Kannan, Vice Chancellor, Thiruvalluvar University for his valuable comments.
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Govindaraju, K., Basha, S.K., Kumar, V.G. et al. Silver, gold and bimetallic nanoparticles production using single-cell protein (Spirulina platensis) Geitler. J Mater Sci 43, 5115–5122 (2008). https://doi.org/10.1007/s10853-008-2745-4
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DOI: https://doi.org/10.1007/s10853-008-2745-4