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Assessment of aneuploidy formation in human blastocysts resulting from donated eggs and the necessity of the embryos for aneuploidy screening

  • Genetics
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Purpose

To examine the prevalence of aneuploidy in human blastocysts resulting from donated eggs and embryo implantation after transfer of normal euploid embryos. Also, to assess the necessity of preimplantation genetic screening (PGS) for embryos produced with donor eggs.

Methods

Blastocysts from donor-recipient cycles were biopsied for PGS (PGS group) and the samples were analyzed with DNA microarray. Euploid blastocysts were transferred to the recipients, and both clinical pregnancy and embryo implantation were examined and compared with embryos without PGS (control group).

Results

After PGS, 39.1 % of blastocysts were abnormal, including aneuploidy and euploid with partial chromosome deletion and/or duplication. Transfer of normal euploid blastocysts brought about 72.4 % of clinical pregnancy, 65.5 % of ongoing/delivery and 54.9 % of embryo implantation rates; these rates were slightly higher than those in the control group (66.7, 54.0 and 47.8 %, respectively), but there was no statistical difference between the two groups. By contrast, the miscarriage rate was higher in the control group (19.2 %) than in the PGS group (9.5 %), but no statistical difference was observed. Transfer of two or more embryos did not significantly increase the ongoing/delivery rates in both groups, but significantly increased the twin pregnancy rates (50.0 % in the PGS group and 43.8 % in the control group).

Conclusion(s)

High proportions of human blastocysts derived from donor eggs are aneuploid. Although pregnancy and embryo implantation rates were increased, and miscarriage rates were reduced by transfer of embryos selected by PGS, the efficiency was not significantly different as compared to the control, suggesting that PGS may be necessary only in some specific situations, such as single embryo transfer.

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Author information

Authors and Affiliations

  1. Houston Fertility Institute, Houston, TX, USA

    Ghassan Haddad, Craig Witz, Daniel Williams, Jason Griffith, Josh Skorupski & Jimmy Gill

  2. Vivere-Houston Fertility Laboratory, Houston, TX, USA

    Cassie T. Wang & Wei-Hua Wang

  3. Vivere Health, Franklin, TN, USA

    Ghassan Haddad, Jimmy Gill & Wei-Hua Wang

  4. Center for Reproductive Medicine, Changsha Hospital for Maternal and Child Health Care, No.416, Chengnan East Road, Changsha City, Hunan, China

    Maimin Deng & Wei-Hua Wang

Authors
  1. Ghassan Haddad
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  2. Maimin Deng
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  3. Cassie T. Wang
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  4. Craig Witz
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  5. Daniel Williams
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  6. Jason Griffith
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  7. Josh Skorupski
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  8. Jimmy Gill
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  9. Wei-Hua Wang
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Corresponding author

Correspondence to Wei-Hua Wang.

Additional information

Capsule Aneuploidy rate is high in human blastocysts resulting from donated eggs and, transfer of normal euploid embryos may facilitate the implementation of single embryo transfer without reducing live birth rate per embryo transfer.

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Haddad, G., Deng, M., Wang, C.T. et al. Assessment of aneuploidy formation in human blastocysts resulting from donated eggs and the necessity of the embryos for aneuploidy screening. J Assist Reprod Genet 32, 999–1006 (2015). https://doi.org/10.1007/s10815-015-0492-4

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  • DOI: https://doi.org/10.1007/s10815-015-0492-4

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