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Luminescence quenching by heavy metal ions of probes based on anthracene, pyrene, and eosin in human serum albumin

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Journal of Applied Spectroscopy Aims and scope

Fluorescence and phosphorescence quenching processes of polar and non-polar luminescent probes associated with human serum albumin (HSA) in phosphate buffer at pH 7.4 were studied. Stern–Volmer quenching constants of anthracene and pyrene fluorescence and eosin phosphorescence and rate constants for quenching of eosin triplet states were determined. The polarity index of pyrene bound to HSA was obtained as a function of thallium nitrate concentration. The influences of structural changes in the proteins that were stimulated by heavy-metal salts and of screening of protein charges by salt ions on quenching processes of singlet and triplet states of the probes were found.

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Authors and Affiliations

  1. Gagarin Saratov State Technical University, 77 Polytechnicheskaya Str., Saratov, 410054, Russia

    E. V. Naumova, A. G. Melnikov & G. V. Melnikov

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  1. E. V. Naumova
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  2. A. G. Melnikov
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  3. G. V. Melnikov
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Correspondence to E. V. Naumova.

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Translated from Zhurnal Prikladnoi Spektroskopii, Vol. 80, No. 2, pp. 165–170, March–April, 2013.

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Naumova, E.V., Melnikov, A.G. & Melnikov, G.V. Luminescence quenching by heavy metal ions of probes based on anthracene, pyrene, and eosin in human serum albumin. J Appl Spectrosc 80, 159–163 (2013). https://doi.org/10.1007/s10812-013-9739-y

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  • DOI: https://doi.org/10.1007/s10812-013-9739-y

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