Abstract
Oomycetes are one of the most serious pathogens that threaten global aquaculture industry, and they cause two out of three most common diseases in Pyropia farms. Recent study showed that Olpidiopsis disease occurring in Pyropia farms in Korea and Japan is caused by different endemic Olpidiopsis species, O. porphyrae in Japan, and O. pyropiae in Korea. From 3 years of epidemiological studies in Pyropia sea farms, we found an additional Olpidiopsis variety causing disease in Pyropia farms in Korea and named it O. porphyrae var. koreanae. It was a clearly different species from previously reported O. pyropiae in 18S rRNA gene sequence (91.0% of identity) and a different variety from Japanese O. porphyrae, which unlike Korean variety had four introns in18S rRNA gene. In our new strain, one more fifth intron was inserted between introns 2 and 3. The sequence similarity of introns ranged from 92.7 to 100%. The ultrastructure, infection process, and asexual life history of this new variety were similar to previously reported Olpidiopsis species, except for the size of zoospores. Phylogenetic analysis using 18S rRNA and cox genes showed that three Pyropia-infecting Olpidiopsis formed a group which had a loose affinity with the other marine Olpidiopsis spp.
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Acknowledgements
This work was supported by a National Research Foundation of Korea Grant (NRF-2015M1A5A1041804) funded to GHK. This research was also supported by Golden Seed Project, Ministry of Agriculture, Food and Rural Affairs funded by Ministry of Oceans and Fisheries, Korea.
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Kwak, M.S., Klochkova, T.A., Jeong, S. et al. Olpidiopsis porphyrae var. koreanae, an endemic endoparasite infecting cultivated Pyropia yezoensis in Korea. J Appl Phycol 29, 2003–2012 (2017). https://doi.org/10.1007/s10811-017-1109-3
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DOI: https://doi.org/10.1007/s10811-017-1109-3