Abstract
Molecular analyses employing sequencing of the complete ribosomal RNA cistron (18S rDNA, ITS1, 5.8S rDNA, ITS2, and 28S rDNA) and transcriptome analysis of the RuBisCO gene (rbcL) were done on Chlorella vulgaris UTEX 2714. The constructed phylogenetic trees showed that C. vulgaris UTEX 2714 is Chlorella sorokiniana. Growth analysis and production of chlorophyll a over a range of increasing cultivation temperatures (27–40 °C) showed that this strain is far less thermotolerant in comparison to a common C. sorokiniana strain. A change in the taxonomic designation of strain UTEX 2714 is proposed.
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Acknowledgments
We thank Raul Llera-Herrera at the Research Center for Food and Development (CIAD, Mazatlan, Mexico) for assembling the transcriptome. At CIBNOR, we thank Paulina Adams and Juan-Pablo Hernandez for technical assistance and microalgae cultivation and Ira Fogel for providing English editing and editorial suggestions. This study was supported by Consejo Nacional de Ciencia y Tecnologia of Mexico (CONACYT-Basic Science-2009, contracts 130656 and 164548) and time for writing by The Bashan Foundation, USA. This is contribution 2015–001 from the Bashan Institute of Science, USA.
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This study is dedicated to the memory of the German/Spanish mycorrhizae researcher Dr. Horst Vierheilig (1964–2011) of CSIC, Spain.
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Fig. S1
Growth and chlorophyll a content of C. sorokiniana UTEX 2805 and strain UTEX 2714 at temperature of 40 °C for 10 days. Values of comparisons of growth of each strain with time denoted by different capital letter differ significantly by one-way ANOVA and LSD post-hoc analysis at p < 0.05. Comparisons between strains in the same sampling time denoted by different lower case letter differ significantly by Student’s t-test at p < 0.05 (PDF 94 kb)
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Bashan, Y., Lopez, B.R., Huss, V.A.R. et al. Chlorella sorokiniana (formerly C. vulgaris) UTEX 2714, a non-thermotolerant microalga useful for biotechnological applications and as a reference strain. J Appl Phycol 28, 113–121 (2016). https://doi.org/10.1007/s10811-015-0571-z
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DOI: https://doi.org/10.1007/s10811-015-0571-z