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Melatonin Inhibits CXCL10 and MMP-1 Production in IL-1β-Stimulated Human Periodontal Ligament Cells

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Abstract

Melatonin is a hormone that is mainly secreted by the pineal gland and exhibits a wide spectrum of activities, including antioxidant functions. Melatonin has been detected in gingival crevicular fluid. However, the role of melatonin in periodontal tissue is still uncertain. The aim of this study was to examine the effects of melatonin on inflammatory mediator expression in human periodontal ligament cells (HPDLC). Interleukin (IL)-1β induced CXC chemokine ligand (CXCL)10, matrix metalloproteinase (MMP)-1, and tissue inhibitors of metalloproteinase (TIMP)-1 production in HPDLC. Melatonin decreased CXCL10 and MMP-1 production and increased TIMP-1 production in IL-1β-stimulated HPDLC. Western blot analysis showed that melatonin inhibited p38 mitogen-activated protein kinase (MAPK) and c-jun N-terminal kinase (JNK) phosphorylation, and IkB-α degradation and phosphorylation in IL-1β-stimulated HPDLC. These results suggest that melatonin might inhibit Th1 cell migration by reducing CXCL10 production. Moreover, melatonin might inhibit soft tissue destruction by decreasing MMP-1 production in periodontal lesions.

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Acknowledgments

This study was supported by Grants-in-Aid for Scientific Research (C) (25463219 and 15K11392), from the Ministry of Education, Culture, Sports, Science and Technology of Japan.

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Authors and Affiliations

  1. Department of Conservative Dentistry, Institute of Biomedical Sciences, Tokushima University Graduate School, School, 3-18-15 Kuramoto-cho, Tokushima, Tokushima, 770-8504, Japan

    Ikuko Hosokawa, Yoshitaka Hosokawa, Satoru Shindo & Takashi Matsuo

  2. Department of Oral Health Care Promotion, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, Tokushima, Japan

    Kazumi Ozaki

Authors
  1. Ikuko Hosokawa
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  2. Yoshitaka Hosokawa
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  3. Satoru Shindo
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  4. Kazumi Ozaki
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  5. Takashi Matsuo
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Correspondence to Yoshitaka Hosokawa.

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Hosokawa, I., Hosokawa, Y., Shindo, S. et al. Melatonin Inhibits CXCL10 and MMP-1 Production in IL-1β-Stimulated Human Periodontal Ligament Cells. Inflammation 39, 1520–1526 (2016). https://doi.org/10.1007/s10753-016-0386-3

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  • DOI: https://doi.org/10.1007/s10753-016-0386-3

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