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Luteolin Prevents LPS-Induced TNF-α Expression in Cardiac Myocytes Through Inhibiting NF-κB Signaling Pathway

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Abstract

Luteolin, a plant flavonoid, has been shown to suppress inflammatory responses; however, the mechanism of luteolin on cardiac myocyte inflammation is still unknown. Because tumor necrosis factor-α (TNF-α), an inflammatory cytokine, is elevated in the failing heart and exerts multiple potentially harmful effects on cardiac myocytes, we therefore sought to examine the effects of luteolin on the expression of TNF-α in neonatal rat cardiac myocytes. In the present study, enzyme-linked immunosorbent assay (ELISA), real-time PCR, immunoblot, immunochemistry staining, and electrophoretic mobility shift assays (EMSA) were performed. ELISA assay showed that luteolin decreased lipopolysaccharide (LPS)-induced production of TNF-α in the medium. Real-time PCR assay confirmed that luteolin also inhibited LPS-induced increase in TNF-α mRNA in myocytes. Furthermore, immunoblot and immunochemistry staining assays represented that luteolin blocked LPS-induced IκB-β degradation and NF-κB p65 subunit nuclear translocation. In addition, EMSA demonstrated that luteolin reduced LPS-induced NF-κB DNA binding activity. Luteolin protects against LPS-induced TNF-α expression via inhibition of the NF-κB signaling pathway, suggesting that luteolin may be a potential therapeutic agent for the treatment of inflammation-related myocardial diseases.

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Abbreviations

LPS:

Lipopolysaccharide

TNF-α:

Tumor necrosis factor-α

DMEM:

Dulbecco’s modified Eagle’s medium

ELISA:

Enzyme-linked immunosorbent assay

NF-κB:

Nuclear factor-κB

PDTC:

Pyrrolidine dithiocarbamate

EMSA:

Electrophoretic mobility shift assays

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ACKNOWLEDGMENTS

This study was based on a project supported by the Scientific Research Fund of Hunan Provincial Education Department (no: 09C048), China. We thank Dr. Tian Lan for the helpful suggestion and discussion during the study.

Conflicts of interest

The authors affirm that they have no conflicts of interest.

Author information

Authors and Affiliations

  1. Department of Pharmacy, Shaoyang Medical College, No.18 West of Baoqing Road, Daxiang District, Shaoyang, 422000, Hunan, China

    Lihua Lv

  2. The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510080, China

    Linhua Lv

  3. Sixth Branch of Internal Medicine, Shaoyang Center Hospital, Shaoyang, 422000, China

    Yubi Zhang

  4. Fourth District of Chemotherapy, Sun Yat-sen University Cancer Center, Guangzhou, 510080, China

    Qiuhuan Kong

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  1. Lihua Lv
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Correspondence to Lihua Lv.

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Lv, L., Lv, L., Zhang, Y. et al. Luteolin Prevents LPS-Induced TNF-α Expression in Cardiac Myocytes Through Inhibiting NF-κB Signaling Pathway. Inflammation 34, 620–629 (2011). https://doi.org/10.1007/s10753-010-9271-7

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