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Distinct Early Inflammatory Events during Ear Tissue Regeneration in Mice Selected for High Inflammation Bearing Slc11a1 R and S Alleles

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Abstract

High inflammatory AIRmax mice homozygous for Slc11a1 R and S alleles were produced. AIRmaxSS mice showed faster ear tissue regeneration than AIRmaxRR mice, suggesting that the S allele favored tissue restoration. Here, we investigated the gene expression profiles and the inflammatory reactions of AIRmaxRR and AIRmaxSS mice during the initial phase of ear tissue regeneration. We observed superior levels of analysis of wound myeloperoxidase and edema in AIRmaxSS mice, although similar cell influx was verified in both lines. Of the genes, 794 were up- and 674 down-regulated in AIRmaxRR, while 735 genes were found to be up- and 1616 down-regulated in AIRmaxSS mice 48 h after punch. Both mouse lines showed significant over-represented genes related to cell proliferation; however AIRmaxSS displayed up-regulation of inflammatory response genes. Quantitative PCR experiments showed higher expressions of Tgfb1, Dap12 and Trem1 genes in AIRmaxSS mice. These results indicate that Slc11a1 gene modulated the early inflammatory events of ear tissue regeneration.

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Acknowledgments

This work was supported by grants from the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) and the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).

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Authors and Affiliations

  1. Laboratório de Imunogenética, Instituto Butantan, São Paulo, SP, Brasil

    Tatiane Canhamero, Luciana C. Peters, Andrea Borrego, Patricia S. Carneiro, Layra L. Albuquerque, Wafa H. Cabrera, Orlando G. Ribeiro, Jose R. Jensen, Nancy Starobinas, Olga M. Ibañez & Marcelo De Franco

  2. Ghost Lab, Laboratory of Cellular and Molecular Immunology, NIAID, NIH, Bethesda, MD, USA

    Brandon Reines

  3. Av. Vital Brasil, 1500, São Paulo, SP, Brazil, 05503900

    Marcelo De Franco

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  1. Tatiane Canhamero
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Correspondence to Marcelo De Franco.

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Canhamero, T., Reines, B., Peters, L.C. et al. Distinct Early Inflammatory Events during Ear Tissue Regeneration in Mice Selected for High Inflammation Bearing Slc11a1 R and S Alleles. Inflammation 34, 303–313 (2011). https://doi.org/10.1007/s10753-010-9235-y

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