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Spatiotemporal expression of caveolin-1 and EMMPRIN during mouse tooth development

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Abstract

Caveolin-1 is a scaffolding protein involved in the formation of cholesterol-rich caveolae lipid rafts within the plasma membrane and is capable of collecting signaling molecules into the caveolae and regulating their activity, including extracellular matrix metalloproteinase inducer (EMMPRIN). However, detailed expression patterns of caveolin-1 and EMMPRIN in the developing dental germ are largely unknown. The present study investigated the expression patterns of caveolin-1 and EMMPRIN in the developing mouse tooth germ by immunohistochemistry and real-time polymerase chain reaction. At the bud stage, caveolin-1 expression was initiated in the epithelium bud and mesenchymal cells, while EMMPRIN was weakly expressed at this stage. At the cap stage, caveolin-1 protein was located in the lingual part of the tooth germ; however, EMMPRIN protein was located in the labial part. From the bell stage to 2 days postnatal, caveolin-1 expression was detected in the ameloblasts and cervical loop area; with EMMPRIN expression in the ameloblasts and odontoblasts. Real-time polymerase chain reaction results showed that both caveolin-1 and EMMPRIN mRNA levels increased gradually with progression of developmental stages, and peaked at day two postnatal. The current finding suggests that both caveolin-1 and EMMPRIN take part in mouse tooth development, especially in the differentiation and organization of odontogenic tissues.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (Grant No. U1204813) and the Key Project of Science and Technology Research of the Education Department of Henan Province (Grant No. 12A320045)

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Authors and Affiliations

  1. Henan Provincial Key Laboratory of Oral Biomedicine, School of Stomatology, Zhengzhou University, 79 Zhongyuandong Road, Zhengzhou, 450000, Henan, People’s Republic of China

    Lu Shi, Lingyun Li & Ding Wang

  2. Shandong Provincial Key Laboratory of Oral Biomedicine, School and Hospital of Stomatology, Shandong University, 44-1 Wenhuaxi Road, Jinan, 250012, Shandong, People’s Republic of China

    Shu Li

  3. Key Lab for Oral Biomedical Engineering, Ministry of Education, School of Stomatology, Wuhan University, Wuhan, People’s Republic of China

    Zhi Chen

  4. Craniofacial Development and Stem Cell Biology, Floor 27 Guy’s Hospital Dental Institute, King’s College London, London, SE1 9RT, UK

    Zhengwen An

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  1. Lu Shi
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  2. Lingyun Li
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  3. Ding Wang
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  4. Shu Li
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Correspondence to Lu Shi.

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Shi, L., Li, L., Wang, D. et al. Spatiotemporal expression of caveolin-1 and EMMPRIN during mouse tooth development. J Mol Hist 47, 337–344 (2016). https://doi.org/10.1007/s10735-016-9675-2

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  • DOI: https://doi.org/10.1007/s10735-016-9675-2

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