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Functional role of EMMPRIN in the formation and mineralisation of dental matrix in mouse molars

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Abstract

Our previous research has shown that the extracellular matrix metalloproteinase inducer (EMMPRIN) is expressed during and may function in the early development of tooth germs. In the present study, we observed the specific expression of EMMPRIN in ameloblasts and odontoblasts during the middle and late stages of tooth germ development using immunohistochemistry. Furthermore, to extend our understanding of the function of EMMPRIN in odontogenesis, we used an anti-EMMPRIN function-blocking antibody to remove EMMPRIN activity in tooth germ culture in vitro. Both the formation and mineralisation of dental hard tissues were suppressed in the tooth germ culture after the abrogation of EMMPRIN. Meanwhile, significant reductions in VEGF, MMP-9, ALPL, ameloblastin, amelogenin and enamelin expression were observed in antibody-treated tooth germ explants compared to control and normal serum-treated explants. The current results illustrate that EMMPRIN may play a critical role in the processing and maturation of the dental matrix.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (Grant No. 30900848), the Science and Technology Commission of Shanghai (Grant No. 09DZ2271100), and the Shanghai Leading Academic Discipline Project (Grant No. T0202).

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Authors and Affiliations

  1. Department of Prosthodontics, Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai, 200011, People’s Republic of China

    Ming Xie, Guofang Xing, Liwen Hou, Jing Bao, Yuqing Chen, Ting Jiao & Fuqiang Zhang

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  1. Ming Xie
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  2. Guofang Xing
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Correspondence to Ting Jiao or Fuqiang Zhang.

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Xie, M., Xing, G., Hou, L. et al. Functional role of EMMPRIN in the formation and mineralisation of dental matrix in mouse molars. J Mol Hist 46, 21–32 (2015). https://doi.org/10.1007/s10735-014-9603-2

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