Skip to main content
SpringerLink
Log in

Retrotransposon insertion variations in doubled haploid bread wheat mutants

  • Original paper
  • Published:
Plant Growth Regulation Aims and scope Submit manuscript

Abstract

The current study attempted to obtain candidate doubled haploid (DH) wheat lines by serially combining two approaches: conventional chemical mutagenesis and anther culture. Additionally, the salt tolerance levels were examined between stress-treated (100 mM NaCl) and non-treated DH groups. For the molecular analysis, IRAP markers were used to characterize retrotransposon insertion polymorphisms induced by haploidization, chromosome doubling, and/or mutagenesis in the DH lines. Various sodium azide (NaN3) concentrations (from 0 to 5 mM) were applied to seeds of the Pehlivan wheat cultivar to obtain an M1 generation mutant population. Anther culture was set up from the M1 mutant population. Green plant regeneration, the frequency of selected candidate mutants within the DH form and the levels of salt tolerance between samples were screened. A total of eight thousand anthers were cultured, and sixteen candidate salt-tolerant DH mutant lines, twenty-seven candidate DH mutant lines with different characteristics and one hundred and two candidate DH lines with morphologically normal appearances were obtained from the NaN3-mutagenized population. The IRAP patterns were quite similar between the control DH lines, and the genetic differences between the controls and DHs originating from possible mutants showed close relatedness. According to previous studies, chemical mutagenesis and anther culture were combined for the first time to detect candidate salt tolerant genotypes at the DH stage. This approach might also be useful for determining the threshold dose and efficiency of wheat mutagens.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  • Al-Qurainy F, Khan S (2009) Mutagenic Effect of sodium azide and its application in crop improvement. World Appl Sci J 6:1589–1601

    CAS  Google Scholar 

  • Anderson JA, Churchill GA, Autrique JE, Tanksley SD, Sorrells ME (1993) Optimizing parental selection for genetic linkage maps. Genome 36:181–186

    Article  CAS  PubMed  Google Scholar 

  • Bonchev G, Georgiev S, Pearce S (2010) Retrotransposons and ethyl methanesulfonate-induced diversity in hexaploid wheat and Triticale. Cent Eur J Biol 5:765–776

    CAS  Google Scholar 

  • Castillo AM, Cistue L, Valles MP, Sanz JM, Romagosa I, Molina-Cano JL (2001) Efficient production of androgenic doubled haploid mutants in barley by the application of sodium azide to anther and microspore cultures. Plant Cell Rep 20:105–111

    Article  CAS  Google Scholar 

  • Cistue L, Soriano M, Castillo AM, Valles MP, Sanz JM, Echavarri B (2006) Production of doubled haploids in durum wheat (Triticum turgidum L.) through isolated microspore culture. Plant Cell Rep 25:257–264

    Article  CAS  PubMed  Google Scholar 

  • Dogramacı-Altuntepe M, Peterson TS, Jauhar PP (2001) Anther culture-derived regenerants of durum wheat and their cytological characterization. J Heredity 92:56–64

    Article  Google Scholar 

  • Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12:13–15

    Google Scholar 

  • Eslami-Farouji A, Khodayari H, Saeidi H, Rahiminejad MR (2015) Genetic diversity of diploid Triticum species in Iran assessed using inter-retroelement amplified polymorphisms (IRAP) markers. Biologia 70/1:52–60

    Google Scholar 

  • Forster BP, Heberle-Bors E, Kasha KJ, Touraev A (2007) The resurgence of haploids in higher plants. Trends Plant Sci 12:368–375

    Article  CAS  PubMed  Google Scholar 

  • Germana MA (2011) Gametic embryogenesis and haploid technology as valuable support to plant breeding. Plant Cell Rep 30:839–857

    Article  CAS  PubMed  Google Scholar 

  • Hasanuzzaman M, Nahar K, Fujita M (2013) Plant response to salt stress and role of exogenous protectants to mitigate salt-induced damages, Ahmad P et al (eds) Ecophysiology and responses of plants under salt stress, Springer Science and Business Media, pp 25–87

  • Hirochika H (1993) Activation of tobacco retrotransposons during tissue culture. EMBO J 12:2521–2528

    CAS  PubMed  PubMed Central  Google Scholar 

  • http://www.naweb.iaea.org/nafa/pbg/public/pbg-nl-35.pdf (2015) Plant Breed Genetics Newslet. 35 2. (Retrived form 13, Jan, 2016)

  • Jain MS (2010) Mutagenesis in crop improvement under the climate change. Rom Biotech Lett 15:88–106

    Google Scholar 

  • Kalendar R (2011) The use of retrotransposon-based molecular markers to analyze genetic diversity. Field Veg Crop Res 48:261–274

    Google Scholar 

  • Kalendar R, Schulman AH (2006) IRAP and REMAP for retrotransposon-based genotyping and fingerprinting. Nat Protoc 1:2478–2484

    Article  CAS  PubMed  Google Scholar 

  • Khan AJ, Tariq SH, Khan T (2001) Haploidy breeding and mutagenesis for drought tolerance in wheat. Euphytica 120:409–414

    Article  Google Scholar 

  • Kovach WL (1999) MVSP-A Multivariate Statistical Package for Windows, v. 3.1. Kovach Computing Services, Pentraeth, pp 133

  • Lee LS, Kim DS, Hyun DY, Lee SJ, Song HS, Lim YP, Lee YI (2003) Isolation of gamma-induced rice mutants with increased tolerance to salt by anther culture. J Plant Biotech 5(1):51–57

    Google Scholar 

  • Lu XP, Gui YJ, Xiao BG, Li YP, Tong ZJ, Liu Y, Bai XF, Wu WR, Xia L, Huttner E, Kilian A, Fan LJ (2013) Development of DArT markers for a linkage map of flue-cured tobacco. Chinese Sci Bull 58(6):641–648

    Article  Google Scholar 

  • Mkuya MS, Si HM, Liu WZ, Sun ZX (2005) Effect of 137Cs gamma rays to panicles on rice anther culture. Rice Sci 12:299–302

    Google Scholar 

  • Munoz M, Cistue L, Castillo AM, Romagosa I, Valles MP (2004) A retrotransposon sequence is related to DNA instability in barley culture, 17th Eucarpia General Congress: genetic variation for plant breeding, Tullin, Austria, 8–11 September 2004

  • Nei M, Li W (1979) Mathematical model for studying genetic variation in terms of restriction endonucleases. P Natl Acad Sci USA 76:5269–5273

    Article  CAS  Google Scholar 

  • Olsen O, Wang X, Von Wetttesin D (1993) Sodium azide mutagenesis: Preferential generation of AT -> GC transitions in the barley Antl8 gene. P Natl Acad Sci USA 90:8043–8047

    Article  CAS  Google Scholar 

  • Powell W, Morgante M, Andre C, Hanafey M, Vogel J, Tingey S, Rafalski A (1996) The comparison of RFLP, RAPD, AFLP SSR (microsatellite) marker for germplasm analysis. Mol Breeding 2:225–238

    Article  CAS  Google Scholar 

  • Prevost A, Wilkinson MJ (1999) A new system of comparing PCR primers applied to ISSR finger printing of potato cultivars. Theor Appl Genet 98:661–668

    Article  Google Scholar 

  • Queen RA, Gribbon BM, James C, Jack P, Flavell AJ (2004) Retrotransposon-based molecular markers for linkage and genetic diversity analysis in wheat. Mol Gen Genomics 271:91–97

    Article  CAS  Google Scholar 

  • Saeidi H, Rahiminejad MR, Heslop-Harrison JS (2008) Retroelement Insertional Polymorphisms, diversity and phylogeography within diploid, D-genome Aegilops tauschii (Triticeae, Poaceae) Sub-taxa in Iran. Ann Bot 101:855–861

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Sikora P, Chawade A, Larsson M, Olsson J, Olsson O (2011) Mutagenesis as a tool in plant genetics, functional genomics, and breeding. Int J Plant Genome. doi:10.1155/2011/314829 (Article: ID 314829)

    Google Scholar 

  • Špunarová M, Ovesná J, Tvarůžek L, Kučera L, Špunar J, Hollerová I (2005) The use of molecular markers for characterisation of spring barley for breeding to Fusarium head blight resistance. Plant Soil Environ 51(11):483–490

    Google Scholar 

  • Tadesse W, Tawkaz S, Inagaki MN, Picard E, Baum M (2013) Methods and Applications of Doubled Haploid Technology in Wheat Breeding. ICARDA, Aleppo, Syria. pp 36

  • Tester M, Langridge P (2010) Breeding technologies to increase crop production in a changing world. Science 327:818–822

    Article  CAS  PubMed  Google Scholar 

  • Vagera J, Novotny J, Ohnoutkova L (2004) Induced androgenesis in vitro in mutated populations of barley, Hordeum vulgare. Plant Cell Tiss Org Cult 77:55–61

    Article  CAS  Google Scholar 

  • Warchoł M, Skrzypek E, Nowakowska A, Marcińska I, Czyczyło-Mysza I, Dziurka K, Juzoń K, Cyganek K (2016) The effect of auxin and genotype on the production of Avena sativa L. doubled haploid lines. Plant Growth Regul 78:155. doi:10.1007/s10725-015-0082-6

    Article  Google Scholar 

Download references

Acknowledgments

This study was supported by The Research Fund of Istanbul University by Project Number 54031 to AS.

Author information

Authors and Affiliations

  1. Department of Biology, Faculty of Science, Istanbul University, 34134, Istanbul, Vezneciler, Turkey

    Ayse Sen

Authors
  1. Ayse Sen
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ayse Sen.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary Table 1 CHB3 media components for androgenic calli induction and R9 (PDF 62 KB)

media components for plantlet regeneration

Supplementary Table 2 PLL media components for plantlet development (PDF 61 KB)

Supplementary Table 3 List of primers used in this study and related information (PDF 63 KB)

10725_2016_209_MOESM4_ESM.pdf

Supplementary Fig. 1 Sukkula-based insertion profiles of the two controls and thirteen candidate DH mutants on a 2% agarose gel (PDF 189 KB)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Sen, A. Retrotransposon insertion variations in doubled haploid bread wheat mutants. Plant Growth Regul 81, 325–333 (2017). https://doi.org/10.1007/s10725-016-0209-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10725-016-0209-4

Keywords

Search

Navigation