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Establishment from the snout and kidney of goldfish, Carassius auratus, of two new cell lines and their susceptibility to infectious pancreatic necrosis virus

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Abstract

Goldfish Carassius auratus are commonly used in scientific research and have a significant economic value in the pet trade. In this study, two cell lines were established from the snout and kidney tissue of goldfish, in order to create a biological monitoring tool for viral diseases. Cell lines were optimally maintained at 25 °C in M199 medium supplemented with 15–20 % fetal bovine serum. Chromosomal analysis indicated that both cell lines remained diploid, with a mean chromosomal count of 100. Results of viral inoculation assays revealed that both cell lines shared similar patterns of viral susceptibility and production to infectious pancreatic necrosis virus (IPNV). The viral titers of IPNV in goldfish snout cell line (GFSe) and goldfish kidney cell line (GFKf) reached 106.8 and 105.9 TCID50/0.1 mL, respectively, within 7 days. The cytopathic effect could be observed when Cyprinid herpesvirus 2 was inoculated into these cells; however, the CPE disappeared after four passages. No CPE was observed in the cells infected by spring viremia carp virus or grass carp reovirus. These newly established cell lines will be a useful diagnostic tool for viral diseases in this fish species and also for the isolation and study of goldfish viruses in future.

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Acknowledgments

The work was supported by the National Key Technology R&D Program (No. 2013BAD12B02) in China.

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Authors and Affiliations

  1. Research Centre of Aquatic Animal Diseases, Institute of Animal Quarantine, Chinese Academy of Inspection and Quarantine, Beijing, 100029, China

    Hongli Jing, Min Zhang, Na Wang, Xiangmei Lin & Shaoqiang Wu

  2. Shekou in Shenzhen Entry-Exit Inspection and Quarantine Bureau, Shenzhen, 518067, China

    Longying Gao

  3. Beijing Entry-Exit Inspection and Quarantine Bureau, Beijing, 100026, China

    Lifeng Zhang

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  1. Hongli Jing
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  2. Longying Gao
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  3. Min Zhang
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  4. Na Wang
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  5. Xiangmei Lin
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  6. Lifeng Zhang
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  7. Shaoqiang Wu
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Correspondence to Shaoqiang Wu.

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Jing, H., Gao, L., Zhang, M. et al. Establishment from the snout and kidney of goldfish, Carassius auratus, of two new cell lines and their susceptibility to infectious pancreatic necrosis virus. Fish Physiol Biochem 42, 303–311 (2016). https://doi.org/10.1007/s10695-015-0138-6

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  • DOI: https://doi.org/10.1007/s10695-015-0138-6

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