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In silico analysis of the regulatory region of the Yellowtail Kingfish and Zebrafish Kiss and Kiss receptor genes

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Abstract

We have cloned and analysed the partial putative promoter sequences of the Yellowtail Kingfish (Seriola lalandi) Kiss2 and Kiss2r genes (380 and 420 bp, respectively). We obtained in silico 1.5 kb of the zebrafish (Danio rerio) Kiss1, Kiss2, Kiss1r and zfKiss2r sequences upstream of the putative transcriptional initiation site. Bioinformatic analysis revealed promoter regulatory elements including AP-1, Sp1, GR, ER, PR, AR, GATA-1, TTF-1, YY1 and C/EBP. These regulatory elements may mediate novel roles of the Kiss genes and their receptors in addition to their established role in reproductive function.

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Acknowledgments

This project was funded by the Australian Seafood CRC (Project No. 2008/745), Fisheries Research and Development Corporation (FRDC) and the University of the Sunshine Coast. We thank Cleanseas Tuna Pty Ltd, South Australia, for providing the Yellowtail Kingfish tissue samples.

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Author notes

  1. A. S. Mechaly

    Present address: Brain and Mind Research Institute, Sydney Medical School, University of Sydney, 100 Mallet Street, Camperdown, NSW, 2050, Australia

Authors and Affiliations

  1. School of Science, Education and Engineering, University of the Sunshine Coast, 90 Sippy Downs Drive, Sippy Downs, QLD, 4556, Australia

    J. N. Nocillado & A. Elizur

  2. Institut de Ciències del Mar, Consejo Superior de Investigaciones Científicas (CSIC), Barcelona, Spain

    A. S. Mechaly

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  1. J. N. Nocillado
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  2. A. S. Mechaly
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  3. A. Elizur
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Correspondence to A. Elizur.

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Nocillado, J.N., Mechaly, A.S. & Elizur, A. In silico analysis of the regulatory region of the Yellowtail Kingfish and Zebrafish Kiss and Kiss receptor genes. Fish Physiol Biochem 39, 59–63 (2013). https://doi.org/10.1007/s10695-012-9642-0

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  • DOI: https://doi.org/10.1007/s10695-012-9642-0

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