Abstract
The Flowering Locus T (FT)-like genes of angiosperms are highly conserved. The FT-encoded proteins include a phosphatidylethanolamine-binding domain that is involved in the control of the shoot apical meristem identity and flowering time. In the present study, FT genes were investigated in 20 bamboo species that are grouped into sympodial, mixed and scattered bamboos based on their morphology. All examined orthologous FT genes consisted of four exons and three introns. Their encoded protein sequences contained the critical amino acid residues Tyr85, Glu109, Leu128, Tyr134, Trp138, Arg139, Gln140 and Asn152, of which each possesses a biological function. The DNA sequences were rich in single nucleotide polymorphism (SNP) sites. The SNP frequency was 1 SNP/16.8 bp, and the nucleotide diversity (π) equaled 0.265. Some SNPs altered restriction enzyme sites or resulted in changes in amino acid contents. The correlation analysis showed that several SNPs were informative in relation to the underground rhizome types of bamboos. Therefore, FT polymorphisms could be used as a tool to identify the underground rhizome types of bamboos. The phylogenetic tree constructed based on the FT gene sequences showed that the obtained clustering was consistent with the underground rhizome types. The SNP markers developed in the present study will provide information on the genetic diversity of bamboos and they can aid taxonomic study as well.
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Acknowledgements
We are grateful to Prof. Helena Korpelainen (Department of Agricultural Sciences, University of Helsinki, Finland) for her critical comments on the manuscript. This work was supported by Grants from the National Natural Science Foundation of China (Grant No. 30901155), the Natural Science Foundation of the Zhejiang Province (Grant No. Y307499), the Pre-research Foundation of the Center for the Cultivation of Subtropical Forest Resources (CCSFR2013001) and Zhejiang Province Preeminence Youth Fund (LR12C16001).
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Xu, Ze., Chen, Hj., Ji, Lf. et al. Polymorphisms of the FT gene as a tool to identify underground rhizome types of bamboos. Euphytica 213, 25 (2017). https://doi.org/10.1007/s10681-016-1824-x
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DOI: https://doi.org/10.1007/s10681-016-1824-x