Abstract
Genetic variation in nitrogen (N) use efficiency, N uptake, and N utilization was analyzed in a doubled haploid (DH) population derived from winter oilseed rape cultivars. The aim was to analyze the relative importance of uptake and utilization efficiency and to identify parameters that allow an easy selection of N efficient genotypes. Fifty-four DH lines were tested in four to seven environments at two levels of N supply: no fertilization and 240 kg N ha−1. N uptake efficiency is defined as the amount of N acquired by a genotype as the proportion of the N available in the soil. N utilization efficiency is measured as unit grain yield per unit of N taken up. Significant genotypic variation was observed for both uptake and utilization efficiency. At low N supply, variation in N efficiency was mainly the result of differences in uptake efficiency. Seed yield was correlated positively with N uptake and N utilization efficiencies at low N supply and with N uptake at high N supply. The correlation was positive between harvest index (HI) and N use efficiency at both N levels (r = 0.45**; r = 0.36**) and for HI and N utilization at low N supply (r = 0.46**), indicating that a shorter plant ideotype might be more N efficient. The interaction between genotypes and N supply for grain yield was highly significant, and the correlation between low N and high N was of only medium size (r = 0.60**), suggesting the possibility of selecting genotypes with specific adaptation to low N supply.
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References
Baligar VC, Duncan RR, Fageria NK (1990) Soil-plant interactions on nutrient use efficiency in plants. In: Baligar VC, Duncan RR (eds) Crops as enhancers of nutrient use. Academic Press, San Diego, pp 352–373
Becker HC, Kazman ME (2000) Genetische Variation für die Stickstoff-Effizienz bei Tritikale. Vort Pflanzenzüchtg 49:111–117
Berry PM, Spink J, Foulkes MJ, White PJ (2010) The physiological basis of genotypic differences in nitrogen use efficiency in oilseed rape (Brassica napus L.). Field Crops Res 119:365–373. doi:10.1016/j.fcr.2010.08.004
Blair GL (1993) Nutrient efficiency: what do we really mean? In: El Bassam N, Dambroth M, Loughman BC (eds) Genetic aspects of plant mineral nutrition. Kluwer, Dordrecht, pp 205–213
Cregan PB, Van Berkum P (1984) Genetics of nitrogen metabolism and physiological/biochemical selection for increased grain crop productivity. Theor Appl Genet 67:97–111
Dhugga KS, Waines JG (1989) Analysis of nitrogen accumulation and use in bead and durum wheat. Crop Sci 29:1232–1239
Gerloff GC (1977) Plant efficiencies in the use of nitrogen, phosphorus, and potassium. In: Wright MJ (ed) Plant adaptation to mineral stress in problem soils. Cornell University Press, New York, pp 161–174
Good AG, Shrawat AK, Muench DG (2004) Can less yield more? Is reducing nutrient input into the environment compatible with maintaining crop production? Trends Plant Sci 9:597–605. doi:10.1016/j.tplants.2004.10.008
Graham RD (1984) Breeding characteristics in cereals. In: Tinker PB, Läuchli A (eds) Advances in Plant Nutrition, vol 1., PraegerNew York, USA, pp 57–90
Henke J, Sieling K, Sauermann W, Kage H (2009) Analysing soil and canopy factors affecting optimum nitrogen fertilization rates of oilseed rape (Brassica napus). J Agric Sci 147:1–8. doi:10.1017/S0021859608008162
Kessel B, Schierholt A, Becker HC (2012) Nitrogen efficiency in a genetically diverse set of winter oilseed rape (Brassica napus L.). Crop Sci 52:2546–2554. doi:10.2135/cropsci2012.02.0134
Lancashire PD, Bleiholder H, Langelüddecke P, Strauss R, Van den Bloom T, Weber E, Witzen-Berger A (1991) A uniform decimal code for growth stages of crops and weeds. Ann Appl Biol 119:561–601
Miersch S, Becker HC (2011) Nitrogen efficiency of different Brassica napus genotypes including semi-dwarf hybrids. Proceedings of the 13th International Rapeseed Congress, June 5–9, Prague, Czech Republic, p 788–792
Moll RH, Kamprath EJ, Jackson WA (1982) Analysis and interpretation of factors which contribute to efficiency of nitrogen utilization. Agron J 74:562–564
Ortiz-Monasterio JI, Sayre KD, Rajaram S, McMahon M (1997) Genetic progress in wheat yield and nitrogen use efficiency under four nitrogen rates. Crop Sci 37:898–904
Ortiz-Monasterio JI, Manske GGB, Van Ginkel M (2001) Nitrogen and phosphorus use efficiency. In: Reynolds MP, Ortiz-Monasterio JI, McNab A (eds) Application of physiology in wheat breeding. CIMMYT, Mexico, pp 200–207
Rathke G-W, Behrens T, Diepenbrock W (2006) Integrated nitrogen management strategies to improve seed yield, oil content and nitrogen efficiency of winter oilseed rape (Brassica napus L.): a review. Agric Ecosyst Environ 117:80–108. doi:10.1016/j.agee.2006.04.006
Reinhardt T-C (1992) Entwicklung und Anwendung von Nah-Infrarot-spektro-skopischen Methoden für die Bestimmung von Öl-, Protein-, Glucosinolat-, Feuchte- und Fettsäure-Gehalten in intakter Rapssaat. Dissertation, Georg August Universität Göttingen, Germany
Sattelmacher B, Horst WJ, Becker HC (1994) Factors that contribute to genetic variation for nutrient efficiency of crop plants. Z Pflanzenernähr Bodenk 157:215–224
Schulte auf’m Erley G, Wijaya KA, Ulas A, Becker HC, Wiesler F, Horst WJ (2007) Leaf senescence and N uptake parameters as selection traits for nitrogen efficiency of oilseed rape cultivars. Physiol Plant 130:519–531. doi:10.1111/j.1399-3054.2007.00921.x
Schulte auf’m Erley G, Behrens T, Ulas A, Wiesler F, Horst WJ (2011) Agronomic traits contributing to nitrogen efficiency of winter oilseed rape. Field Crops Res 124:114–123. doi:10.1016/j.fcr.2011.06.009
Sieling K, Kage H (2008) The potential of semi-dwarf oilseed rape genotypes to reduce the risk of N leaching. J Agric Sci 146:77–84
Sylvester-Bradley R, Kindred DR (2009) Analyzing nitrogen responses of cereals to prioritize routes to the improvement of nitrogen use efficiency. J Exp Bot 60:1939–1951. doi:10.1093/jxb/erp116
Ulas A, Behrens T, Wiesler F, Horst WJ, Schulte auf’m Erley G (2013) Does genotypic variation in nitrogen remobilisation efficiency contribute to nitrogen efficiency of winter oilseed-rape cultivars (Brassica napus L.). Plant Soil 371:463–471. doi:10.1007/s11104-013-1688-y
Utz HF (2011) Plabstat. Ein Computerprogramm zur statistischen Analyse von pflanzenzüchterischen Experimenten. Version 2 N. Institut für Pflanzenzüchtung und Populationsgenetik, Universität Hohenheim, Germany
Van Sanford DA, MacKown CT (1986) Variation in nitrogen use efficiency among soft red winter wheat genotypes. Theor Appl Genet 72:158–163
VDLUFA (1991) Verband Deutscher Landwirtschaftlicher Untersuchungs- und Forschungsanstalten (VDLUFA). Methodenhandbuch Band 1. Die Untersuchung von Boden. 4th edn. VDLUFA, Darmstadt
Velasco L, Möllers C (2000) Use of Near-Infrared Reflectance Spectros-copy to assess the nitrogen concentration in different plant tissues of rape-seed. Commun Soil Sci Plant Anal 31:2987–2996
Acknowledgments
The work was supported by Deutsche Forschungsgemeinschaft (DFG). We thank Dr. Jürgen Koch and Landmännen SW Seed for providing the DH population ‘Apex × Mohican’ and for performing field trials in Teendorf.
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Nyikako, J., Schierholt, A., Kessel, B. et al. Genetic variation in nitrogen uptake and utilization efficiency in a segregating DH population of winter oilseed rape. Euphytica 199, 3–11 (2014). https://doi.org/10.1007/s10681-014-1201-6
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DOI: https://doi.org/10.1007/s10681-014-1201-6