Abstract
This study was carried out to find out the comparative distribution of heavy metals (Fe, Cu, Mn, Zn, Co, Cr, Cd and Pb) in various tissues (muscles, gills, liver, stomach and intestine) of Cyprinus carpio from Rawal Lake, Pakistan, during summer and winter. Relatively higher concentrations of Cd, Co, Cr, Cu, Fe and Zn were found in the stomach samples, while the highest Pb and Zn levels were noted in muscle and intestine samples, respectively. Correlation study exhibited diverse relationships among the metals in various tissues. Generally, the metal concentrations found during the summer were comparatively higher than the winter. Potential non-carcinogenic and carcinogenic health risks related to the metals in C. carpio were evaluated using the US Environmental Protection Agency approved cancer risk assessment guidelines. The calculated daily and weekly intakes of Pb, Cd, Cr and Co through the fish consumption were significantly higher than the permissible limits. In relation to the non-carcinogenic risks to human, Pb, Cd, Cr, Co and Zn levels were higher than the safe limits; however, carcinogenic risks related to Cr (3.9 × 10−3 during summer and 1.1 × 10−3 during winter) and Pb (2.6 × 10−4 during summer and 1.5 × 10-4 during winter) clearly exceeded the safe limit (1 × 10−6). Consequently, the consumption of C. carpio from Rawal Lake on regular basis was considered unsafe.
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Alam, M. G. M., Tanaka, A., Allinson, G., Laurenson, L. J. B., Stagnitti, F., & Snow, E. T. (2002). A comparison of trace element concentrations in cultured and wild carp (Cyprinus carpio) of Lake Kasumigaura, Japan. Ecotoxicol Environ Saf, 53, 348–354.
Bratakos, M. S., Lazos, E. S., & Bratakos, S. M. (2002). Chromium content of selected Greek foods. Science of the Total Environment, 290, 47–58.
Burger, J., Gaines, K. F., Boring, C. S., Stephens, W. L., Snodgrass, J., Dixon, C., McMahon, M., Shukla, S., Shukla, T., & Gochfeld, M. (2002). Metal levels in fish from the Savannah River: Potential hazards to fish and other receptors. Environ Res, 89, 85–97.
Calabrese, E. J., Canada, A. T., & Sacco, C. (1985). Trace elements and public health. Annual Reviews of Public Health, 6, 131–146.
Carvalho, M. L., Santiago, S., & Nunes, M. L. (2005). Assessment of the essential element and heavy metal content of edible fish muscle. Anal Bioanal Chem, 382, 426–432.
CEC (2001). Commission Regulation (EC) No. 221/2002 of February 6, 2002 amending regulation (EC) no. 466/2002 setting maximum levels for certain contaminants in foodstuffs. Official Journal of the European Communities, Commission of the European Communities, Brussels.
Celik, U., & Oehlenschlager, J. (2007). High contents of cadmium, lead, zinc and copper in popular fishery products sold in Turkish supermarkets. Food Control, 18, 258–261.
Choongo, K. C., Syakalima, M. S., & Mwase, M. (2005). Coefficient of condition in relation to copper levels in muscle of serranochromis fish and sediment from the Kafue River, Zambia. Bull Environ Contam Toxicol, 75, 645–651.
Costa, S. D., & Hartz, S. M. (2009). Evaluation of trace metals (cadmium, chromium, copper and zinc) in tissues of a commercially important fish (Leporinus obtusidens) from Guaiba Lake, Southern Brazil. Brazilian Archives of Biology and Technology, 52, 241–250.
Dural, M., Goksu, M. Z. L., & Ozak, A. A. (2007). Investigation of heavy metal levels in economically important fish species captured from the Tuzla lagoon. Food Chem, 102, 415–421.
EC (European Communities) (2001). Commission Regulation as regards heavy metals, Directive 2001/22/EC, No. 466/2001 EEC as amended by regulation 221/2002/EC.
Erdogrul, O., & Erbilir, F. (2007). Heavy metal and trace elements in various fish samples from Sir Dam Lake, Kahramanmaraş, Turkey. Environ Monit Assess, 130, 373–379.
FAO (1983). Compilation of legal limits for hazardous substances in fish and fishery products. FAO Fishery Circular No. 464, 5–10. Rome: Food and Agriculture Organization of the United Nations
FAO/WHO (2002). Human vitamin and mineral requirements, Report of a joint Food and Agriculture Organization of the United Nations/World Health Organization expert consultation, Bangkok, Thailand. http://www.fao.org/documents/show_cdr.asp?url_file=/DOCREP/004/Y2809E/y2809e00.htm.
FAO/WHO (2004). Summary of evaluations performed by the joint FAO/WHO expert committee on food additives (JECFA 1956–2003). First through sixtyfirst meetings. Washington: ILSI Press, International Life Sciences Institute
Ikem, A., & Egiebor, N. O. (2005). Assessment of trace elements in canned fishes (mackerel, tuna, salmon, sardines and herrings) marketed in Georgia and Alabama (United States of America). Journal of Food Composition and Analysis, 18, 771–787.
Iram, S., Ahmad, I., Ahad, K., Muhammad, A., & Anjum, S. (2009). Analysis of pesticides residues of Rawal and Simly lakes. Pakistan Journal of Botany, 41, 1981–1987.
Jones, B. R., & Laslett, R. E. (1994). Methods for analysis for trace metals in marine and other samples, aquatic environment protection: Analytical methods (pp. 1–29). Lowestoft: Ministry of Agriculture Fisheries and Food, Directorate of Fisheries Research.
Karadede, H., & Unlu, E. (2000). Concentrations of some heavy metals in water, sediment and fish species from the Ataturk Dam Lake (Euphrates), Turkey. Chemosphere, 41, 1371–1376.
Kucuksezgin, A., Altay, O., Uluurhan, E., & Kontas, A. (2001). Trace metal and organochlorine residue levels in red mullet (Mullus barbatus) from the Eastern Aegean, Turkey. Water Res, 35, 2327–2332.
Lin, M. C. (2009). Risk assessment on mixture toxicity of arsenic, zinc and copper intake from consumption of milkfish, Chanos chanos (Forsskal), cultured using contaminated groundwater in Southwest Taiwan. Bull Environ Contam Toxicol, 83, 125–129.
MAFF (2000). Monitoring and surveillance of non-radioactive contaminants in the aquatic environment and activities regulating the disposal of wastes at sea, 1997. Aquatic environment monitoring report number 52. Lowestoft: Ministry of Agriculture, Fisheries and Food (MAFF)
Matta, J., Milad, M., Manger, R., & Tosteson, T. (1999). Heavy metals, lipid peroxidation and cigateratoxicity in the liver of the Caribbean barracuda (Sphyraena barracuda). Biol Trace Elem Res, 70, 69–79.
Ozturk, M., Ozozen, G., Minareci, O., & Minareci, E. (2009). Determination of heavy metals in fish, water and sediments of Avsar Dam Lake in Turkey. Iranian Journal of Environmental Health Science and Engineering, 6, 73–8073.
Pak-EPA (2004). Report on Rawal lake catchment area monitoring operation. Ministry of Environment, Islamabad, Pakistan Environmental Protection Agency
Qiao-Qiao, C., Guang-wei, Z., & Langdon, A. (2007). Bioaccumulation of heavy metals in fishes from Taihu Lake, China. J Environ Sci, 19, 1500–1504.
Rashed, M. N. (2001). Monitoring of environmental heavy metals in fish from Nasser Lake. Environ Int, 27, 27–33.
Shah, A. Q., Kazi, T. G., Arain, M. B., Baig, J. A., Afridi, H. I., Kandhro, G. A., Sumaira Khan, S., & Jamali, M. K. (2009). Hazardous impact of arsenic on tissues of same fish species collected from two ecosystems. Journal of Hazardous Material, 167, 511–515.
Sivaperumal, P., Sankar, T. V., & Nair, P. G. V. (2007). Heavy metal concentrations in fish, shellfish and fish products from internal markets of India vis-a-vis international standards. Food Chem, 102, 612–620.
Tekin-Ozan, S., & Kir, I. (2006). Concentrations of some heavy metals in organs of two fish species from the Beysehir Lake, Turkey. Fresenius Environmental Bulletin, 15, 530–534.
Tekin-Ozan, S., & Kir, I. (2008). Seasonal variations of heavy metals in some organs of carp (Cyprinus carpio L., 1758) from Beysehir Lake (Turkey). Environ Monit Assess, 138, 201–206.
Turkmen, M., Turkmen, A., Tepe, Y., Tore, Y., & Ates, A. (2009). Determination of metals in fish species from Aegean and Mediterranean Seas. Food Chem, 113, 233–237.
Tuzen, M. (2003). Determination of heavy metals in fish samples of the middle Black Sea (Turkey) by graphite furnace atomic absorption spectrometry. Food Chem, 80, 119–123.
Tuzen, M. (2009). Toxic and essential trace element contents in fish species from the Black Sea, Turkey. Food Chem Toxicol, 47, 1785–1790.
USEPA (1983). Methods for chemical analysis of water and waste. EPA Report 600/4-79-020. Cincinnati: Office of Water, Unites States Environmental Protection Agency
USEPA (2003). Technical standard operating procedure, SOP EH-06, adopted from draft ERT/REAC SOP for fish collection. East Helena Site, Montana: Unites States Environmental Protection Agency
USEPA (2006). USEPA region III risk-based concentration table: Technical background information. Unites States Environmental Protection Agency.
USEPA (2011). Risk-based concentration table. http://www.epa.gov/reg3hwmd/risk/human/index.htm.
Vinodhini, R., & Narayanan, M. (2008). Bioaccumulation of heavy metals in organs of fresh water fish Cyprinus carpio (Common carp). International Journal of Environmental Science and Technology, 5, 179–182.
Walker, C. H., Hopkin, S. P., Sibly, R. M., & Peakall, D. B. (2001). Principles of ecotoxicology (2nd ed., p. 309). London: Taylor and Francis.
Ward, S. M., & Neumann, R. M. (1999). Seasonal variations in concentrations of mercury in axial muscle tissue of largemouth bass. North American Journal of Fisheries Management, 19, 89–96.
Watanabe, K. H., Desimone, F. W., Thiyagarajah, A., Hartley, W. R., & Hindrichs, A. E. (2003). Fish tissue quality in the lower Mississippi River and health risks from fish consumption. Science of the Total Environment, 302, 109–126.
WHO (1996). Health criteria other supporting information. In Guidelines for drinking water quality, 2nd edn (pp. 31–388). Geneva: World Health Organization
Yilmaz, A. B., Sangun, M. K., Yaglioglu, D., & Turan, C. (2010). Metals (major, essential to non-essential) composition of the different tissues of three demersal fish species from Iskenderun Bay, Turkey. Food Chem, 123, 410–415.
Yilmaz, F. (2006). Bioaccumulation of heavy metals in water, sediment, aquatic plants and tissues of Cyprinus carpio from Kizilirmak, Turkey. Fresenius Environmental Bulletin, 15, 360–369.
Zhao, S., Feng, C., Quan, W., Chen, X., Niu, J., & Shen, Z. (2012a). Role of living environments in the accumulation characteristics of heavy metals in fishes and crabs in the Yangtze River Estuary, China. Mar Pollut Bull, 64, 1163–1171.
Zhao, S., Feng, C., Yang, Y., Niu, J., & Shen, Z. (2012b). Risk assessment of sedimentary metals in the Yangtze Estuary: New evidence of the relationships between two typical index methods. J Hazard Mater, 241–242, 164–172.
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The research fellowship awarded by Quaid-i-Azam University, Islamabad, Pakistan to carry out this project is thankfully acknowledged.
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Iqbal, J., Shah, M.H. Study of seasonal variations and health risk assessment of heavy metals in Cyprinus carpio from Rawal Lake, Pakistan. Environ Monit Assess 186, 2025–2037 (2014). https://doi.org/10.1007/s10661-013-3515-6
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DOI: https://doi.org/10.1007/s10661-013-3515-6