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Morphological and molecular characterisation of Ditylenchus stenurus n. sp. (Nematoda: Anguinidae) from western Iran

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Abstract

A new species in the genus Ditylenchus, D. stenurus n. sp. collected from western Iran, is described and illustrated herein based on morphological and molecular studies. The new species is characterised by a body length of 772 (663–863) μm, delicate stylet 6 (5–7) μm long, six lines in the lateral field. Median bulb of pharynx well-developed, muscular with crescentic valve. Post-vulval uterine sac well-developed, 35 (30–45) μm long, female tail elongate-conoid, becoming narrow suddenly with finely rounded terminus. The new species comes close in morphology and morphometrics to five known species of the genus, namely D. arachis, D. caudatus, D. clarus, D. myceliophagus, and D. nanus. DNA sequencing data was obtained on the partial 18S, D2/D3 expansion segments of the 28S rRNA gene and internal transcribed spacer (ITS). The phylogenetic relationships of this species with other Ditylenchus spp. using partial 18S–rDNA and D2/D3 indicated that D. stenurus n. sp. clustered together with several species belongs to the D. triformis-group i. e. D. africanus, D. destructor and D. halictus: all sharing a rounded tail terminus and six lines in lateral fields.

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Notes

  1. The species epithet refers to the morphology of the female tail (Greek words, στενός = narrow, and ουρά = tail)

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Acknowledgements

The authors thank the University of Tehran for financial support and Mr. Mahdi Esmaeili for his help during sampling.

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Authors and Affiliations

  1. Department of Plant Protection, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

    Mehrab Esmaeili & Ramin Heydari

  2. Faculty of Agriculture, Roudehen branch, Islamic Azad University (IAU), Roudehen, Iran

    Mozhgan Ziaie

  3. Nematode Assay Section, Agronomic Division, North Carolina Department of Agriculture and Consumer Services, Raleigh, NC, 27607, USA

    Weimin Ye

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  1. Mehrab Esmaeili
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Correspondence to Ramin Heydari.

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Esmaeili, M., Heydari, R., Ziaie, M. et al. Morphological and molecular characterisation of Ditylenchus stenurus n. sp. (Nematoda: Anguinidae) from western Iran. Eur J Plant Pathol 149, 533–542 (2017). https://doi.org/10.1007/s10658-017-1201-1

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