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Occurrence and molecular variability of Potato spindle tuber viroid and Tomato apical stunt viroid in ornamental plants in Croatia

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Abstract

Viroids of the genus Pospiviroid are able to induce diseases in a wide range of host plants including important crop species. Although occasional disease outbreaks of Potato spindle tuber viroid (PSTVd) and closely related pospiviroids have been reported in potato and tomato, recent studies found an increase in number of latent infections in ornamental solanaceous species. In order to verify the presence of PSTVd and other pospiviroids in Croatia, a survey was conducted between 2009 and 2012. A total of 182 samples belonging to five ornamental species and two solanaceous crops were analyzed. Eight plants belonging to two different species (Solanum jasminodes and Lycianthes rantonnetii) were found infected by PSTVd and, in addition, one S. jasminoides plant infected by Tomato apical stunt viroid (TASVd). Viroid infection was confirmed by mechanical inoculation on tomato plants to observe symptom expression. Molecular characterization of the isolates was done and complete viroid sequences were submitted to the GenBank. This is the first evidence of the presence of PSTVd and TASVd and their variability in Croatia.

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Acknowledgements

This research was funded by the Croatian Ministry of Agriculture.

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Authors and Affiliations

  1. Croatian Centre for Agriculture, Food and Rural Affairs, Institute for Plant Protection, Rim 98, 10000, Zagreb, Croatia

    Jasna Milanović & Vesna Kajić

  2. Institute Ruđer Bošković, Bijenička cesta 54, 10000, Zagreb, Croatia

    Snježana Mihaljević

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  1. Jasna Milanović
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  2. Vesna Kajić
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  3. Snježana Mihaljević
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Correspondence to Jasna Milanović.

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Milanović, J., Kajić, V. & Mihaljević, S. Occurrence and molecular variability of Potato spindle tuber viroid and Tomato apical stunt viroid in ornamental plants in Croatia. Eur J Plant Pathol 139, 785–788 (2014). https://doi.org/10.1007/s10658-014-0432-7

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