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Effects of sanitation processes on survival of Synchytrium endobioticum and Globodera rostochiensis

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Abstract

The two quarantine pests Synchytrium endobioticum, the causal agent of potato wart disease and Globodera rostochiensis, the yellow potato cyst nematode are currently present in Germany. Winter sporangia of Synchytrium endobioticum and cysts of Globodera rostochiensis can be spread with waste from potato processing industries, if infected tubers are processed. The German Biowaste Ordinance prescribes sanitation of organic waste before it can be used on arable land as fertilizer or filling material. Sanitation parameters prescribed by the German Biowaste Ordinance include composting for 7 days at 65°C or 14 days at 55°C or pasteurisation for 60 min at 70°C. The effect of composting and pasteurisation processes on winter sporangia of Synchytrium endobioticum and cysts of Globodera rostochiensis was tested with varying time-temperature relations. Cysts of Globodera rostochiensis were killed by composting for 7 days at 50–55°C and by pasteurisation for 30 min at 70°C. In contrast, viable winter sporangia of Synchytrium endobioticum could be extracted from sample material after composting for 70 days at 30–45°C, composting for 21 days at 50–55°C and after composting for 12 days at 60–65°C. Likewise viable winter sporangia could be extracted after pasteurisation for 90 min at 70°C and heating in a water bath at 80°C and in a dry oven at 90°C for 8 h. The parameters prescribed in the German Biowaste Ordinance are sufficient to kill cysts of Globodera rostochiensis but not sufficient to kill winter sporangia of Synchytrium endobioticum in organic waste.

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Abbreviations

EPPO:

European and Mediterranean Plant Protection Organisation

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Acknowledgements

This research was funded by the Federal Ministry of Food, Agriculture and Consumer Protection.

The support of Prof. Dr. G. Busch and M. Sieber from the Brandenburg Technical University Cottbus, Chair in Waste Management, with the technical conduction of composting and pasteurisation, of Dr. D. Heinicke, Dr. S. Krüssel and H. Warnecke from the Plant Protection Service of Lower Saxony by providing viable cysts of G. rostochiensis and testing of cysts viability and the support of the companies Emsland-Stärke GmbH Kyritz and FRIWEIKA for providing potato waste is gratefully acknowledged.

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Authors and Affiliations

  1. Julius Kühn-Institut, Federal Research Centre for Cultivated Plants, Institute for National and International Plant Health, Stahnsdorfer Damm 81, 14532, Kleinmachnow, Germany

    Silke Steinmöller & Petra Müller

  2. Faculty of Agriculture and Horticulture, Department of Crop and Animal Sciences, Phytomedicine, Humboldt-Universität zu Berlin, Lentzeallee 55-57, 14195, Berlin, Germany

    Martina Bandte & Carmen Büttner

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  1. Silke Steinmöller
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Correspondence to Silke Steinmöller.

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Steinmöller, S., Bandte, M., Büttner, C. et al. Effects of sanitation processes on survival of Synchytrium endobioticum and Globodera rostochiensis . Eur J Plant Pathol 133, 753–763 (2012). https://doi.org/10.1007/s10658-012-9955-y

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