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Association of Grapevine fanleaf virus, Tomato ringspot virus and Grapevine rupestris stem pitting-associated virus with a grapevine vein-clearing complex on var. Chardonnay

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Abstract

A disease complex was observed on grapevine var. Chardonnay (Vitis vinifera) in a commercial vineyard in Missouri that destroyed the affected vineyard. Conspicuous vein-clearing symptoms on the leaves of originally diseased Chardonnay vines and bud-grafted Chardonnay, V. vinifera ‘Cabernet Franc’, V. vinifera ‘Baco Blanc’, and hybrid ‘LN-33’ vines are characteristics of the disease complex, which is referred to as the grapevine vein-clearing complex (GVCC). By applying reverse-transcription polymerase chain reaction (RT-PCR) using virus-specific primers, we detected combinations of Grapevine fanleaf virus (GFLV), Tomato ringspot virus (ToRSV) and Grapevine rupestris stem pitting-associated virus (GRSPaV), in symptomatic Chardonnay vines. Sequencing of RT-PCR amplified DNA fragments confirmed the identity of each virus, indicating the occurrence of ToRSV yellow vein strain, and two distinct strains of GRSPaV in the GVCC-affected Chardonnay vines. This is the first report of the co-infection of two nepoviruses and GRSPaV in var. Chardonnay. This study demonstrated that mixed infections of grapevine viruses belonging to different taxonomic groups pose a great threat to vineyards under certain climatic and soil conditions.

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Acknowledgements

We thank Karen Scholthof, Marilyn Odneal and Patrick Winterhagen for critical review of the manuscript. We thank Keith Striegler for informing us about this disease, and the owner and manager of the Chardonnay vineyard for their permission and assistance in sample collection. We extend our appreciation to Adib Rowhani, University of California at Davis, for providing PCR-amplified ToRSV fragments and primer sequences, and to Ruth Welliver at the Pennsylvania Department of Agriculture for providing ToRSV-infected Chenopodium quinoa. This project was supported by funding from the Missouri Wine and Grape Board and from USDA-Viticulture Consortium-East Section under the subaward number 51401-8279 through Cornell University.

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Authors and Affiliations

  1. Department of Agriculture, Missouri State University, 9740 Red Spring Road, Mountain Grove, MO, 65711, USA

    Shaista Lunden, John Avery Jr. & Wenping Qiu

  2. Department of Molecular and Cellular Biology, University of Guelph, 50 Stone Road East, Guelph, ON, N1G 2W1, Canada

    Baozhong Meng

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  1. Shaista Lunden
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  2. Baozhong Meng
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  4. Wenping Qiu
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Correspondence to Wenping Qiu.

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Lunden, S., Meng, B., Avery, J. et al. Association of Grapevine fanleaf virus, Tomato ringspot virus and Grapevine rupestris stem pitting-associated virus with a grapevine vein-clearing complex on var. Chardonnay. Eur J Plant Pathol 126, 135–144 (2010). https://doi.org/10.1007/s10658-009-9527-y

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  • DOI: https://doi.org/10.1007/s10658-009-9527-y

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