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Identification and characterization of a potyvirus causing chlorotic spots on Phalaenopsis orchids

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Abstract

A putative virus-induced disease showing chlorotic spots on leaves of Phalaenopsis orchids was observed in central Taiwan. A virus culture, phalaenopsis isolate 7-2, was isolated from a diseased Phalaenopsis orchid and established in Chenopodium quinoa and Nicotiana benthamiana. The virus reacted with the monoclonal antibody (POTY) against the potyvirus group. Potyvirus-like long flexuous filament particles around 12–15 × 750–800 nm were observed in the crude sap and purified virus preparations, and pinwheel inclusion bodies were observed in the infected cells. The conserved region of the viral RNA was amplified using the degenerate primers for the potyviruses and sequence analysis of the virus isolate 7-2 showed 56.6–63.1% nucleotide and 44.8–65.1% amino acid identities with those of Bean yellow mosaic virus (BYMV), Beet mosaic virus (BtMV), Turnip mosaic virus (TuMV) and Bean common mosaic virus (BCMV). The coat protein (CP) gene of isolate 7-2 was amplified, sequenced and found to have 280 amino acids. A homology search in GenBank indicated that the virus is a potyvirus but no highly homologous sequence was found. The virus was designated as Phalaenopsis chlorotic spot virus (PhCSV) in early 2006. Subsequently, a potyvirus, named Basella rugose mosaic virus isolated from malabar spinach was reported in December 2006. It was found to share 96.8% amino acid identity with the CP of PhCSV. Back-inoculation with the isolated virus was conducted to confirm that PhCSV is the causal agent of chlorotic spot disease of Phalaenopsis orchids in Taiwan. This is the first report of a potyvirus causing a disease on Phalaenopsis orchids.

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Acknowledgements

We are grateful to Dr. Wen-Hsiung Ko, Emeritus Professor of University of Hawaii at Manoa, and V. Panwar for critically reviewing and editing the manuscript. We thank Dr. Hei-Ti Hsu’s valuable discussion and Mr. Wen-Fua Ko’s technical assistance. This study was partially supported by a grant (94AS-13.3.1-BQ-B1) from the Bureau of Animal and Plant Health Inspection and Quarantine, Council of Agriculture, Executive Yuan, Taiwan, R.O.C.

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Authors and Affiliations

  1. Department of Plant Pathology, National Chung Hsing University, Taichung, 402, Taiwan

    You-Xiu Zheng, Yuh-Kun Chen & Fuh-Jyh Jan

  2. Taichung District Agriculture Improvement Station, Changhua, 515, Taiwan

    Ching-Chung Chen

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  1. You-Xiu Zheng
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  2. Ching-Chung Chen
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Correspondence to Fuh-Jyh Jan.

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Zheng, YX., Chen, CC., Chen, YK. et al. Identification and characterization of a potyvirus causing chlorotic spots on Phalaenopsis orchids. Eur J Plant Pathol 121, 87–95 (2008). https://doi.org/10.1007/s10658-008-9281-6

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