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Ecotoxicological evaluation of foundry sands and cosmetic sludges using new earthworm biomarkers

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Abstract

The management and final disposal of industrial wastes are a matter of considerable human concern. The present study evaluates the cyto/genotoxic effects and changes of the coelomic cell formulas exerted by aqueous leachates and solid waste (SW) of two industrial residues using coelomocytes extruded from Eisenia fetida. The assayed wastes corresponded to industrial foundry and cosmetic activities. After 14 days of exposure, we obtained a group of endpoints that reflect the toxicity/genotoxicity, coelomocyte formula and indexes; and the mortality classical value (LC50-14d). Among the variables measured, total coelomocytes formula (eleocytes + amebocytes + granulocytes) appears as a single and easy parameter to assess the toxicity of eluates at short exposure times. We applied a set of assays using earthworms as test organism that would allow evaluating SW as well as its aqueous leachates. It is easy to run trials combining exposures of 1 h to 14 days, which can be integrated into the implementation of the traditional test for evaluating acute toxicity.

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References

  • Adamowicz A (2005) Morphology and ultrastructures ofmthe earthworm Dendrobaena veneta (Lumbricidae) coelomocytes. Tissue Cell 37:125–133

    Article  CAS  Google Scholar 

  • Adamowicz A, Wojtaszek A (2001) Morphology and phagocytotic activity of coelomocytes in Dendrobaena veneta (Lumbricidae). Zool Pol 46:91–104

    Google Scholar 

  • APHA-AWWA-WPCF (1998) In: Franson M (ed) Standard methods for the examination of water and wastewater, 20th edn. American Public Association, American Water Works Association, Water Environment Federation, Washington

    Google Scholar 

  • Bogacki J, Naumczyk J, Marcinowski P, Kucharska M (2011) Treatment of cosmetic wastewater using physicochemical and chemical methods. Chemik 65(2):94–97

    CAS  Google Scholar 

  • Brulle F, Mitta G, Cocquerelle C, Vieau D, Lemiere S, Lepretre A, Vandenbulcke F (2006) Cloning and real-time PCR testing of 14 potential biomarkers in Eisenia fetida following cadmium exposure. Environ Sci Technol 40:2844–2850

    Article  CAS  Google Scholar 

  • Calisi A, Lionetto MG, Schettino T (2011) Biomarker response in the earthworm Lumbricus terrestris exposed to chemical pollutants. Sci Total Environ 409:4456–4464

    Article  CAS  Google Scholar 

  • Carnin RL, Folgueras MV, Luvizao RR, Correia SL, da Cunha CJ, Dungan RS (2012) Use of an integrated approach to characterize the physicochemical properties of foundry green sands. Thermochim Acta 543:150–155

    Article  CAS  Google Scholar 

  • Carter MR (1993) Soil sampling and methods of analysis. Lewis Publishers, Boca Raton

    Google Scholar 

  • CEN 14735 (2005) Characterization of waste – preparation of waste samples for ecotoxicity tests. European Standard, Brussels, Belgium

    Google Scholar 

  • Cooper EL (1996) Earthworm immunity. In: Rinkevich B, Muller EG (eds) Invertebrate inmmunology. Springer-Verlag, Berlin, pp 10–45

    Chapter  Google Scholar 

  • Cotelle S, Ferard JF (1999) Comet assay in genetic ecotoxicology: a review. Environ Mol Mutagen 34:246–255

    Article  CAS  Google Scholar 

  • Deprez K, Robbens J, Nobels I, Vanparys C, Vanermen G, Tirez K, Michiels L, Weltens R (2012) DISCRISET: a battery of tests for waste classification. Application of tests on waste extracts. Waste Manage 32:2218–2228

    Article  CAS  Google Scholar 

  • Di Marzio WD, Sáenz ME, Lemière S, Vasseur P (2005) Improved single-cell electrophoresis assay for detecting DNA damage in Eisenia foetida. Environ Mol Mut 46:246–252

    Article  Google Scholar 

  • Di Marzio WD, Sáenz ME, Montivero C, Alberdi JL, Tortorelli MC, Ambrini G (2007) Genotoxicity of acqueous elutions of industrial soils. Bull Environ Contam Toxicol 79(5):483–487

    Article  Google Scholar 

  • Dungan RS, Huweb J, Chaney RL (2009) Concentrations of PCDD/PCDFs and PCBs in spent foundry sands. Chemosphere 75:1232–1235

    Article  CAS  Google Scholar 

  • Edwards PJ, Coulson JM (1992) Choice of earthworm species for laboratory tests. In: Becker H et al (eds) Ecotoxicology of earthworms. Intercept, Hants, pp 36–43

    Google Scholar 

  • Esplugas S, Bila DM, Krause LG, Dezotti M (2007) Ozonation and advanced oxidation technologies to remove disrupting chemicals (EDCs) and pharmaceuticals and personal care products (PPCPs) in water effluents. J Hazard Mater 149:631–642

    Article  CAS  Google Scholar 

  • Eyambe SG, Gaven AJ, Fitzpatrick LC, Venables BJ, Cooper EL (1991) A non-invasive technique for sequential collection of earthworm (Lumbricus terrestris) leukocytes during subchronic immunotoxicity studies. Lab Anim 25:61–67

    Article  CAS  Google Scholar 

  • Han Y, Zhu L, Wang J, Wang J, Xie H, Zhang S (2014) Integrated assessment of oxidative stress and DNA damage in earthworms (Eisenia fetida) exposed to azoxystrobin. Ecotoxicol Environ Saf 107:214–219

    Article  CAS  Google Scholar 

  • Homa J, Niklinska M, Plytycz B (2003) Effect of heavy metals on coelomocytes of the earthworm Allolobophora chlorotica. Pedobiología 47:640–645

    CAS  Google Scholar 

  • International Standard Organization (1998) Soil quality-effects of pollutants on earthworms (Eisenia fetida)—Part 2: Determination of effects on reproduction. ISO 11268-2, Switzerland

    Google Scholar 

  • Kasprzyk-Hordern B, Dinsdale RM, Guwy AJ (2009) The removal of pharmaceuticals, personal care products, endocrine disruptors and illicit drugs during wastewater treatment and its impact on the quality of receiving waters. Water Res 43:363–380

    Article  CAS  Google Scholar 

  • Końca K, Lankoff A, Banasik A, Lisowska H, Kuszewski T, Gózd S, Koza Z, Wojcik A (2003) A cross platform public domain PC image analysis program for the comet assay. Mutat Res 534:15–20

    Article  Google Scholar 

  • Kostka-Rick R (2004) Ecotoxicological characterization of waste – Method development for determining the ecotoxicological (H14) risk criterion. Landesanstalt für Umweltschutz Baden-Württemberg, Germany

    Google Scholar 

  • Kumarravel TS, Jha AN (2006) Reliable Comet assay measurements for detecting DNA damage induced by ionising radiation and chemicals. Mutat Res 605:7–16

    Article  Google Scholar 

  • Kurek A, Homa J, Kauschke E, Plytycz B (2007) Characteristics of coelomocytes of the stubby earthworm, Allolobophora chlorotica (Sav.). Eur J Soil Biol 43:121–126

    Article  Google Scholar 

  • Mazur AI, Klimek M, Morgan AJ, Plytycz B (2011) Riboflavin storage in earthworm chloragocytes and chloragocyte-derived eleocytes and its putative role as chemoattractant for immunocompetent cells. Pedobiologia 54:37–42

    Article  Google Scholar 

  • Miguel RE, Ippolito JA, Leytem AB, Porta AA, Noriega RB, Dungan RS (2012) Analysis of total metals in waste molding and core sands from ferrous and non-ferrous foundries. J Environ Manage 110:77–81

    Article  CAS  Google Scholar 

  • Miguel RE, Ippolito JA, Porta AA, Noriega RB, Dungan RS (2013) Use of standardized procedures to evaluate metal leaching from waste foundry sands. J Environ Qual 42:615–620

    Article  CAS  Google Scholar 

  • Moser H, Kessler H (2009) Ecotoxicological characterization of waste as an instrument in waste classification and risk assessment. In: Moser H, Römbke J (eds) Ecotoxicological characterization of waste. Springer, New York, pp 281–290

    Chapter  Google Scholar 

  • Moser H, Römbke J (2009) Ecotoxicological characterization of waste. Results and experiences of an European ring test. Springer Ltd., New York 308 pp

    Book  Google Scholar 

  • Moser H, Roembke J, Donnevert G, Becker R (2011) Evaluation of biological methods for a future methodological implementation of the Hazard criterion H14 ‘ecotoxic’ in the European waste list (2000/532/EC). Waste Manage Res 29(2):180–187

    Article  CAS  Google Scholar 

  • OECD (2000) Guidelines for testing of chemicals: earthworm reproduction test Eisenia fetida. OECD, Paris

    Google Scholar 

  • Olchawa E, Bzowska M, Stürzembaum S, Morgan J, Plytycz B (2006) Heavy metals affect the coelomocyte-bacteria balance in earthworms: environmental interactions between abiotic and biotic stressors. Environ Pollut 142:373–381

    Article  CAS  Google Scholar 

  • OPDS (2006) Provincial Law of Special Wastes. Toxic wastes under Res. N° 1532. http://www.opds.gba.gov.ar/index.php/leyes/ver/236. Accessed 22 May 2014

  • Pandard P, Römbke J (2013) Proposal for a “harmonized” strategy for the assessment of the HP 14 property. Int Environ Assess Manage 9:665–672

    Article  Google Scholar 

  • Pandard P, Devillers J, Charissou AM, Poulsen V, Jourdain MJ, Férard JF, Grand C, Bispo A (2006) Selecting a battery of bioassays for ecotoxicological characterization of wastes. Sci Total Environ 363:114–125

    Article  CAS  Google Scholar 

  • Quiao M, Chen Y, Wang CH, Wang Z, Zhu Y (2007) DNA damage and repair process in earthworm after in vivo and in vitro exposure to soils irrigated by wastewaters. Environ Pollut 148:141–147

    Article  Google Scholar 

  • Rand GM (1995) Fundamentals of aquatic toxicology. Taylor & Francis, Washington

    Google Scholar 

  • R Core Team (2013) R: a language and environment for statistical computing. R Foundation for Statistical Computing, Vienna. ISBN 3-900051-07-0. http://www.R-project.org

  • Reif R, Suárez SD, Omil FG, Lema JM (2008) Fate of pharmaceuticals and cosmetics ingredients during the operation of a MBR treating sewage. Desalination 221:511–517

    Article  CAS  Google Scholar 

  • Römbke J, Moser H, Moser T (2009) Overview on the results of the ring test. In: Moser H, Römbke J (eds) Ecotoxicological characterization of waste. Springer, New York, pp 3–26

    Chapter  Google Scholar 

  • SADSN (1992). Hazardous waste law 24.051. http://www.ambiente.gov.ar/?aplicacion=normativa&IdNorma=147&IdSeccion=22. Accessed 22 May 2014

  • Sparks T (2000) Statistics in ecotoxicology. Wiley, New York

    Google Scholar 

  • Statistica (2007) StatSoft Inc (data analysis software system), version 8.0. www.statsoft.com

  • US EPA (1991) Ecological Assessment of hazardous waste sites: a field and laboratory reference. Washington EPA/540/R-92/003. http://www.epa.gov/region9/qa/pdfs/fieldsamp-ecologicalassess.pdf. Accessed 4 May 2015

  • US EPA (2007) Test methods for evaluating solid waste, physical/chemical methods SW846. Method 3550C, Ultrasonic extraction. http://www.epa.gov/osw/hazard/testmethods/sw846/pdfs/3550c.pdf. Accessed 13 May 2015

  • Volpe MG, Nazzaro M, Coppola R, Rapuano F, Aquino P (2012) Determination and assessments of selected heavy metals in eye shadow cosmetics from China, Italy, and USA. Microchem J 101:65–69

    Article  CAS  Google Scholar 

  • Wang J, Cao X, Chai L, Liao J, Huang Y, Tang X (2015) Oxidative damage of naphthenic acids on the Eisenia fetida earthworm. Environ Toxicol. doi:10.1002/tox.22139

    Google Scholar 

  • West Inc (1996) Toxstat Version 3.5. Cheyenne, WY

  • Wilke B, Riepert F, Koch C, Kuhne T (2008) Ecotoxicological characterization of hazardous wastes. Ecotoxicol Environ Saf 70:283–293

    Article  CAS  Google Scholar 

  • Zar JH (2010) Biostatistical analysis. Pearson, Boston

    Google Scholar 

Download references

Acknowledgments

This research was supported by National University of Luján, Buenos Aires, and National Scientific and Technical Research Council, Argentina.

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Authors and Affiliations

  1. National Scientific and Technical Research Council CONICET, Av. Rivadavia 1917, 1023, Buenos Aires, Argentina

    Silvana Patricia Curieses, María Elena Sáenz, Marcelo Larramendy & Walter Di Marzio

  2. Ecotoxicology Research Program, Department of Basic Sciences, National University of Luján, 221, B6700, Luján, Buenos Aires, Argentina

    Silvana Patricia Curieses, María Elena Sáenz & Walter Di Marzio

  3. Faculty of Natural Sciences and Museum, National University of La Plata, Calle 64 Nº 3, B1904AMA, La Plata, Argentina

    Marcelo Larramendy

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  1. Silvana Patricia Curieses
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  2. María Elena Sáenz
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  4. Walter Di Marzio
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Correspondence to Walter Di Marzio.

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Curieses, S.P., Sáenz, M.E., Larramendy, M. et al. Ecotoxicological evaluation of foundry sands and cosmetic sludges using new earthworm biomarkers. Ecotoxicology 25, 914–923 (2016). https://doi.org/10.1007/s10646-016-1649-3

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