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NAFLD Induction Delays Postoperative Liver Regeneration of ALPPS in Rats

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Abstract

Background

Associating liver partition and portal vein ligation (ALPPS) is a promising two-step hepatectomy that is beneficial for accumulative regeneration of the future liver remnant (FLR) and avoids postoperative liver failure.

Aims

Our study aimed to evaluate whether nonalcoholic fatty liver disease affected the liver regeneration induced by ALPPS.

Methods

Sprague-Dawley rats fed a high-fat diet were used to construct the NAFLD model. ALPPS were performed, and blood and future liver remnant samples were collected at postoperative days 1 (POD1), POD3, and POD7.

Results

The hepatic regeneration rate (HRR) of ALPPS was higher than that of portal vein ligation (PVL) at POD3 and POD7 (p < 0.05), and the number of Ki-67-positive hepatocytes (POD3) and CD68-positive Kupffer cells (POD7) per visual field was higher in the ALPPS group than in the PVL group (p < 0.05). The serum TNF-α, hepatocyte growth factor protein, and the serum IL-6 level were higher in the ALPPS group than in the PVL group at POD3 and POD7. Compared with those of the standard laboratory diet (SLD)-fed rats, the rats with NAFLD exhibited a decrease in the HRR, Ki-67-positive hepatocytes, and CD68-positive Kupffer cells in the FLR. The number of CD68-positive Kupffer cells was lower in rats with NAFLD than that in SLD-fed rats; noteworthily, the serum level of IL-6 and TNF-α changed dramatically after surgeries.

Conclusions

NAFLD induction delayed liver regeneration induced by the ALPPS procedure, which might be associated with hepatocyte proliferation and the number of Kupffer cells.

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Abbreviations

FLR:

Portal vein ligation

SLD:

Standard laboratory diet

HGF:

Hepatocyte growth factor

LML:

Left median lobe

LLL:

Left lateral lobe

PLF:

Postoperative liver failure

PBE:

Portal branch embolization

TCHO:

Total cholesterol

ALPPS:

Associating liver partition and portal vein ligation

NAFLD:

Nonalcoholic fatty liver disease

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Acknowledgments

This work was supported by the Fund for Science and Technology Development of Jilin Province (20160101060JC, 20150101108JC); the National Key R&D Program of China (2017YFD0502200, 2016YFD0501302); the Project of the Education Department of Jilin Province (No. 2016444).

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Authors and Affiliations

  1. Department of Hepatopancreatobiliary Surgery of Second Hospital of Jilin University, Key Laboratory for Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun, 130041, China

    Jinwei Zhao, Hongyue Xu, Yuan Li, Lulu Gong, Ge Zheng, Xuefei Wang, Wenjin Luan, Shulin Li, Fangxue Ma, Lihui Ni & Lu Yu

  2. Key Lab for New Drugs Research of TCM in Shenzhen, Research Institute of Tsinghua University in Shenzhen, Shenzhen, 518057, China

    Xudong Tang & Xueyan Wang

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  1. Jinwei Zhao
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  2. Hongyue Xu
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  3. Yuan Li
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Correspondence to Lu Yu.

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Zhao, J., Xu, H., Li, Y. et al. NAFLD Induction Delays Postoperative Liver Regeneration of ALPPS in Rats. Dig Dis Sci 64, 456–468 (2019). https://doi.org/10.1007/s10620-018-5346-3

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  • DOI: https://doi.org/10.1007/s10620-018-5346-3

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