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Isolation and characterization of microsatellite markers for the heavily exploited rockfish Sebastes schlegeli, and cross-species amplification in four related Sebastes spp.

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Abstract

Here we report development and characterization of 14 polymorphic microsatellite loci from Sebastes schlegel. Polymorphism at these loci revealed from 3 to 23 alleles. The observed heterozygosity ranged from 0.34 to 1.00, and the expected heterozygosity ranged from 0.31 to 0.95. No linkage disequibrium was found. Two loci were significantly deviated from HWE (P < 0.01). The 14 loci were also surveyed in four other Sebastes species and 12 loci successfully amplified, where allelic diversity ranged from highly polymorphic to monomorphic. These results demonstrate these microsatellite markers can be used for the study of intra- and inter-specific genetic diversity.

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Acknowledgments

This work is funded by a grant from the National Fisheries Research and Development (NFRDI) and is contribution number, RP-09-BT-004. Views expressed herein are those of the authors and do not necessarily reflect the views of NFRDI.

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Authors and Affiliations

  1. Biotechnology Research Institute, National Fisheries Research and Development Institute, Busan, 619-705, Korea

    Hye Suck An, Jung Youn Park, Mi-Jung Kim, Eun Young Lee & Kyung Kil Kim

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  1. Hye Suck An
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  2. Jung Youn Park
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  3. Mi-Jung Kim
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  4. Eun Young Lee
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  5. Kyung Kil Kim
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Correspondence to Hye Suck An.

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An, H.S., Park, J.Y., Kim, MJ. et al. Isolation and characterization of microsatellite markers for the heavily exploited rockfish Sebastes schlegeli, and cross-species amplification in four related Sebastes spp.. Conserv Genet 10, 1969–1972 (2009). https://doi.org/10.1007/s10592-009-9870-8

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  • DOI: https://doi.org/10.1007/s10592-009-9870-8

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