Abstract
We developed 29 forensic quality tetranucleotide microsatellite loci for the identification of individual black bear (Ursus americanus). Allele number averaged 5.35 and ranged from 3 to 11. The eight markers selected for forensic use permit the identification and exclusion of individual bear with a probability of identity of approximately 8.1 × 10−8 (approximately one per 12 million).
References
Butler J (2001) Forensic DNA typing. Academic Press. San Diego, California, USA
Hanson DG (1983) Poaching: oriental demand for ungdam and paws decimates California Bears. Audubon 85(4):127–128
Inman K, Rudin N (2002) An introduction to forensic DNA analysis, 2nd edn. CRC Press, Boca Raton, Florida, USA
Jones KC, Levine KF, Banks JD (2000) DNA-based genetic markers in black-tailed and mule deer for forensic applications. Calif Fish and Game 86:115–126
Paetkau D, Calvert W, Stirling I, Strobeck C (1995) Microsatellite analysis of population structure in Canadian polar bears. Mol Ecol 4:347–354
Raymond M, Rousset F (1995) GENEPOP (version 1.2): population genetics software for exact tests and ecumenicism. J Hered 86:248–249
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning, a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, New York, USA
Updike D, Bernard L (2004) All around California, responsible people can help keep bears wild. In: DFG News. http://www.dfg.ca.gov/news/news04/04069.html Accessed 3 Mar 2008
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Meredith, E.P., Rodzen, J.A., Banks, J.D. et al. Characterization of 29 tetranucleotide microsatellite loci in black bear (Ursus americanus) for use in forensic and population applications. Conserv Genet 10, 693–696 (2009). https://doi.org/10.1007/s10592-008-9617-y
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10592-008-9617-y