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Identification and characterization of microsatellites from the Antarctic isopod Ceratoserolis trilobitoides: nuclear evidence for cryptic species

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Abstract

We report the successful isolation of 10 polymorphic microsatellite markers for one species of the marine isopod species complex Ceratoserolis trilobitoides (Eights, 1833) from the Southern Ocean. The number of alleles per locus ranged from 6 to 30 for the 148 specimens analysed and heterozygosity ranged from 0.34 to 0.98. Seven microsatellites amplified successfully in a cryptic sister species, which is restricted to the Antarctic Peninsula. This novel marker set provides the opportunity to study and monitor population structure, demography and gene flow patterns for a benthic model taxon in a region that is now subject to rapid climate change.

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Acknowledgements

We thank Wolfgang Wägele (Museum Koenig, Bonn, Germany) and Tilman Alpermann (AWI Bremerhaven, Germany) for helpful discussions and Andrea Eschbach for technical assistance. Anna Kop (York University, Toronto, Canada) proofread an earlier version of the manuscript. This work was supported by a DFG grant HE-3391/3 to Christoph Held.

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Authors and Affiliations

  1. Alfred Wegener Institute for Polar and Marine Research, P.O. Box 12 0161, 27515, Bremerhaven, Germany

    Florian Leese & Christoph Held

  2. Department of Animal Ecology, Evolution and Biodiversity, Ruhr-University of Bochum, Universitaetsstrasse 150, 44780, Bochum, Germany

    Florian Leese

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  1. Florian Leese
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  2. Christoph Held
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Correspondence to Florian Leese.

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Leese, F., Held, C. Identification and characterization of microsatellites from the Antarctic isopod Ceratoserolis trilobitoides: nuclear evidence for cryptic species. Conserv Genet 9, 1369–1372 (2008). https://doi.org/10.1007/s10592-007-9491-z

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  • DOI: https://doi.org/10.1007/s10592-007-9491-z

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