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Up-Regulation of TAB3 Is Involved in Neuronal Apoptosis After Intracerebral Hemorrhage

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Abstract

Human transforming growth factor β-activated kinase (TAK1)-binding protein 3 (TAB3) is a regulator of NF-κB which has been mainly found in a variety of cancers. While TAB3 is highly expressed in brain tissue, little is known about the function of TAB3 in central nervous system. Our group established an animal ICH model with autologous whole blood injected into brain, and also a cell ICH model with hemin stimulation. Our Western blot result showed up-regulation of TAB3 during neuronal apoptosis in the model of intracerebral hemorrhage (ICH), which was also approved by immunofluorescence and immunohistochemistry result. Besides, increasing TAB3 level was accompanied by the increased expression of active-caspase-3, active-caspase-8, and decreased expression of Bcl-2. Furthermore, in in vitro study, the level of neuronal apoptosis was decreased by applying TAB3- RNA interference in PC12 cells. All the results above suggested that TAB3 probably participates in the process of neuronal apoptosis following ICH.

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Acknowledgments

Grant sponsor: The National Natural Science Foundation of China (Nos. 81471188, 81371299).

Author Contributions

Liang Zhu, Maohong Cao, Yaohui Ni, Lijian Han, Aihua Dai, Rongrong Chen, Xiaojin Ning and Xiaorong Liu did all the experiments; Kaifu Ke provided the data and wrote the manuscript.

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    Authors and Affiliations

    1. Department of Neurology, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, People’s Republic of China

      Liang Zhu, Maohong Cao, Yaohui Ni, Lijian Han, Aihua Dai, Rongrong Chen, Xiaojin Ning, Xiaorong Liu & Kaifu Ke

    2. Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, Nantong, 226001, Jiangsu, People’s Republic of China

      Liang Zhu, Lijian Han & Aihua Dai

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    1. Liang Zhu
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    Correspondence to Kaifu Ke.

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    Liang Zhu and Maohong Cao contributed equally to this work.

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    Zhu, L., Cao, M., Ni, Y. et al. Up-Regulation of TAB3 Is Involved in Neuronal Apoptosis After Intracerebral Hemorrhage. Cell Mol Neurobiol 37, 607–617 (2017). https://doi.org/10.1007/s10571-016-0397-5

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