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Infections in the tissue material and their impact on the loss of transplants in the Laboratory of in vitro Cell and Tissue Culture with Tissue Bank in the years 2011–2015

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Abstract

Radiation sterilization eliminates microbiological infections but causes the degradation of the cell factor. The negative result of microbiological examination for tissue transplants is one of the conditions for approval for distribution in patients. The study attempts to verify impact of the presence of microbes onto material for transplant loss. In the 2011–2015 period, we analyzed 293 donors of skin and amnion. Microbiological sampling was performed. The total of 21 strains of bacteria, molds and fungi was identified in collected tissue. The widest spectrum of strains was found in skin (17), followed by amnia (8). The total number of positive findings was 147 and was again highest in skin (129), while the number of positive findings in amnia was 18 only. The general percentage of fungal infections was very low. The presence of fungal strains was only observed in allogeneic skin (2%). Large number of microorganisms isolated from the skin before sterilization was observed, so it seems impossible to use allogeneic intravital skin. However, the intravital application of allogeneic amnion obtained from cesarean section remains to be considered.

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Authors and Affiliations

  1. Dr Stanisław Sakiel Center for Burns Treatment, Jana Pawła II 2, 41-100, Siemianowice Śląskie, Poland

    D. Kitala, A. Klama-Baryła, M. Kawecki, M. Kraut, W. Łabuś, J. Glik, M. Ples & M. Nowak

  2. Department of Chronic Wounds Management Organization, School of Health Sciences in Katowice, Medical University of Silesia, Katowice, Poland

    J. Glik

  3. Department of Health Sciences, Technical-Humanistic Academy, Willowa 2, 43-309, Bielsko-Biała, Poland

    M. Kawecki

  4. Non-Public Laboratory of Microbiology, Jana Pawła II 2, 41-100, Siemianowice Śląskie, Poland

    E. Tomanek

Authors
  1. D. Kitala
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  2. A. Klama-Baryła
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  3. M. Kawecki
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  4. M. Kraut
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  5. W. Łabuś
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  6. J. Glik
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  7. M. Ples
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  8. E. Tomanek
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  9. M. Nowak
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Correspondence to D. Kitala.

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Kitala, D., Klama-Baryła, A., Kawecki, M. et al. Infections in the tissue material and their impact on the loss of transplants in the Laboratory of in vitro Cell and Tissue Culture with Tissue Bank in the years 2011–2015. Cell Tissue Bank 18, 61–68 (2017). https://doi.org/10.1007/s10561-016-9597-y

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  • DOI: https://doi.org/10.1007/s10561-016-9597-y

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