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Retina-on-a-chip: a microfluidic platform for point access signaling studies

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Abstract

We report on a microfluidic platform for culture of whole organs or tissue slices with the capability of point access reagent delivery to probe the transport of signaling events. Whole mice retina were maintained for multiple days with negative pressure applied to tightly but gently bind the bottom of the retina to a thin poly-(dimethylsiloxane) membrane, through which twelve 100 μm diameter through-holes served as fluidic access points. Staining with toluidine blue, transport of locally applied cholera toxin beta, and transient response to lipopolysaccharide in the retina demonstrated the capability of the microfluidic platform. The point access fluidic delivery capability could enable new assays in the study of various kinds of excised tissues, including retina.

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Acknowledgments

This material is based upon work supported by the following grants 1) National Eye Institute - RO1EY020496-01 (RMS) and P30EY08126 (Vanderbilt Vision Research Center), 2) Research to Prevent Blindness, Inc. - Unrestricted Grant (Vanderbilt Eye Institute) and Career Development Award (RMS), and 3) National Science Foundation Graduate Research Fellowship Program under Grant No. 0909667 and 1445197.

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Authors and Affiliations

  1. Department of Mechanical Engineering, Vanderbilt University, Nashville, TN, USA

    Kirsten H. Dodson, Deyu Li & Jon F. Edd

  2. Department of Ophthalmology and Visual Sciences, Vanderbilt Eye Institute, Vanderbilt University School of Medicine, Nashville, TN, USA

    Franklin D. Echevarria & Rebecca M. Sappington

  3. Cancer Center and BioMEMS Resource Center, Massachusetts General Hospital, Boston, MA, USA

    Jon F. Edd

Authors
  1. Kirsten H. Dodson
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  2. Franklin D. Echevarria
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  3. Deyu Li
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  4. Rebecca M. Sappington
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  5. Jon F. Edd
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Correspondence to Deyu Li.

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Dodson, K.H., Echevarria, F.D., Li, D. et al. Retina-on-a-chip: a microfluidic platform for point access signaling studies. Biomed Microdevices 17, 114 (2015). https://doi.org/10.1007/s10544-015-0019-x

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