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Integrated microfluidic biophotonic chip for laser induced fluorescence detection

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Abstract

Integrated Lab-on-a-Chip or Micro-Total Analysis Systems offer several advantages for the detection of active chemical and biological species. In this work, an integrated microfluidic biophotonic chip is proposed for carrying out laser induced fluorescence detection. A Spectrometer-on-Chip device, specifically designed for multiple fluorescence detections at different emission wavelengths is integrated with the opto-microfluidic chip fabricated on Silicon-Polymer hybrid platform. The input fiber from the laser source, and output fiber coupled with a Spectrometer-on-Chip were integrated with the microfluidic channel so as to make a robust setup. Fluorescence detection was carried out using Alexafluor 647 tagged antibody particles. The experimental results show that the proposed biophotonic microfluidic device is highly suitable for high throughput detection of chemical and biological specimens.

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Acknowledgement

The authors would like to thank the Canadian Institute for Photonic Innovations (CIPI) and Enablence Inc. for their support in this project. The authors also sincerely thank Mr. Eric Duchesne of Ecole Polytechnique de Montreal for his assistance with the Scanning Electron Microscopy and Mr. Donald Walter Berry of McGill University for his assistance with wafer dicing.

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Authors and Affiliations

  1. Optical Bio Microsystems Laboratory, Department of Mechanical Engineering, Concordia University, Montreal, QC, Canada

    Arvind Chandrasekaran & Muthukumaran Packirisamy

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  1. Arvind Chandrasekaran
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  2. Muthukumaran Packirisamy
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Correspondence to Arvind Chandrasekaran.

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Chandrasekaran, A., Packirisamy, M. Integrated microfluidic biophotonic chip for laser induced fluorescence detection. Biomed Microdevices 12, 923–933 (2010). https://doi.org/10.1007/s10544-010-9447-9

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