Abstract
We describe the fabrication of three-dimensional tissue constructs using a magnetic force-based tissue engineering technique, in which cellular organization is controlled by magnetic force. Target cells were labeled with magnetite cationic liposomes (MCLs) so that the MCL-labeled cells could be manipulated by applying a magnetic field. Line patterning of human umbilical vein endothelial cells (HUVECs) labeled with MCLs was successfully created on monolayer cells or skin tissues using a magnetic concentrator device. Multilayered cell sheets were also inducible on a culture surface by accumulating MCL-labeled cells under a uniform magnetic force. Based on these results, we attempted to construct a complex multilayered myoblast C2C12 cell sheet. Here, patterned HUVECs were embedded by alternating the processes of magnetic accumulation of C2C12 cells for cell layer formation and magnetic patterning of HUVECs on the cell layers. This technique may be applicable for the fabrication of complex tissue architectures required in tissue engineering.
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Acknowledgments
We thank Toda Kogyo Co. for supplying the magnetite. This work was supported in part by Grants-in-Aid for Scientific Research (nos. 19686049 and 20034043) from the Japan Society for the Promotion of Science (JSPS).
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Akiyama, H., Ito, A., Kawabe, Y. et al. Fabrication of complex three-dimensional tissue architectures using a magnetic force-based cell patterning technique. Biomed Microdevices 11, 713–721 (2009). https://doi.org/10.1007/s10544-009-9284-x
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DOI: https://doi.org/10.1007/s10544-009-9284-x