Skip to main content
SpringerLink
Log in

Response of Arabidopsis thaliana root growth to phosphorus and its relation to media chemical composition

  • Original paper
  • Published:
Biologia Plantarum

Abstract

The interaction between phosphorus (P) and other media components alters root development and masks the plant response and thus limits the ability to correctly identify P-deficiency response (pdr) mutants. This study aims to assess changes in root development caused by different composition of growth media normally used in Arabidopsis research and to study their effects on pdr-mutant screening. Primary root growth of four genotypes was analyzed in media differing in P concentrations: half-strength Murashige and Skoog (½ MS) and Somerville and Ogren (SO). The effects of nitrogen source and Fe on root growth were investigated in each medium separately and in a mixture. We found that the primary root length of all genotypes grown on ½ MS was reduced in comparison with plants grown on SO medium. The mutant pdr9 was the most sensitive in ½ MS, This mutant was also hypersensitive to Fe that intensified its sensitivity to ammonium. Ammonium increased the root inhibition caused by Fe also in wild-type plants. In conclusion, on the basis of our study we recommend to use SO medium, which ensures an efficient selection to screen for pdr mutants through root growth. Moreover, nitrogen sources in the media other than nitrate should be taken carefully.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

MS:

Murashige and Skoog

pdr1 :

phosphate deficiency response1

PHR1:

phosphate starvation response 1

QC:

quiescent center

SO:

Somerville and Ogren

WT:

wild type

References

  • Abercrombie, J., Halfhill, M., Ranjan, P., Rao, M., Saxton, A., Yuan, J., Stewart, C.N.: Transcriptional responses of Arabidopsis thaliana plants to As(v) stress. — BMC Plant Biol. 8: 87, 2008.

    Article  PubMed  PubMed Central  Google Scholar 

  • Babourina, O., Voltchanskii, K., McGann, B., Newman, I., Rengel, Z.: Nitrate supply affects ammonium transport in canola roots. — J. exp. Bot. 58: 651–658, 2007.

    Article  CAS  PubMed  Google Scholar 

  • Briat, J.-F., Rouached, H., Tissot, N., Gaymard, F., Dubos, C.: Integration of P, S, Fe and Zn nutrition signals in Arabidopsis thaliana: potential involvement of phosphate starvation response 1 (phr1). — Front.Plant Sci. 6: 290, 2015.

    Article  PubMed  PubMed Central  Google Scholar 

  • Britto, D.T., Kronzucker, H.J.: NH4 + toxicity in higher plants: a critical review. — J. Plant Physiol. 159: 567–584, 2002.

    Article  CAS  Google Scholar 

  • Cerutti T., Delatorre C.A.: Nitrogen and phosphorus interaction and cytokinin responses of the primary root of Arabidopsis thaliana and the pdr1 mutant. — Plant Sci. 198: 91–97, 2013.

    Article  CAS  PubMed  Google Scholar 

  • Chen, D.L., Delatorre, C.A., Bakker, A., Abel, S.: Conditional identification of phosphate-starvation-response mutants in Arabidopsis thaliana. — Planta 211: 13–22, 2000.

    Article  CAS  PubMed  Google Scholar 

  • Chevalier, F., Pata, M., Nacry, P., Doumas, P., Rossignol, M.: Effects of phosphate availability on the root system architecture: large-scale analysis of the natural variation between Arabidopsis accessions. — Plant Cell Environ. 26: 1839–1850, 2003.

    Article  CAS  Google Scholar 

  • Costa, C.T., Strieder, M.L., Abel, S., Delatorre, C.A.: Phosphorus and nitrogen interaction: loss of QC identity in response to P or N limitation is antecipated in pdr23 mutant. — Braz. J. Plant Physiol. 23: 219–229, 2011.

    Article  CAS  Google Scholar 

  • Delatorre, C.A.: Phosphate Deficiency Response: Searching for the Signaling Pathway. — Lambert Academic Publishing, Köln, Germany 2009.

    Google Scholar 

  • Feng, J., Volk, R.J., Jackson, W.A.: Source and magnitude of ammonium generation in maize roots. — Plant Physiol. 118: 835–841, 1998.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Garnica, M., Houdusse, F., Zamarreño, A.M., Garcia-Mina, J.M.: The signal effect of nitrate supply enhances active forms of cytokinins and indole acetic acid content and reduces abscisic acid in wheat plants grown with ammonium. — J. Plant Physiol. 167: 1264–1272, 2010.

    Article  CAS  PubMed  Google Scholar 

  • González-Mendoza, V., Zurita-Silva, A., Sánchez-Calderón, L., Sánchez-Sandoval, M.E., Oropeza-Aburto, A., Gutiérrez, A.D., Alatorre-Cobos, F., Herrera-Estrella, L.: Apsr1, a novel gene required for meristem maintenance, is negatively regulated by low phosphate availability. — Plant Sci. 205–206: 2–12, 2013.

    Article  PubMed  Google Scholar 

  • Hammond, J.P., White, P.J.: Sucrose transport in the phloem: integrating root responses to phosphorus starvation. — J. exp. Bot. 59: 93–109, 2008.

    Article  CAS  PubMed  Google Scholar 

  • Hammond, J.P., White, P.J.: Sugar signaling in root responses to low phosphorus availability. — Plant Physiol. 156: 1033–1040, 2011.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Hirsch, J., Marin, E., Floriani, M., Chiarenza, S., Richaud, P., Nussaume, L., Thibaud, M.C.: Phosphate deficiency promotes modification of iron distribution in Arabidopsis plants. — Biochimie 88: 1767–1771, 2006.

    Article  CAS  PubMed  Google Scholar 

  • Jain, A., Poling, M.D., Smith, A.P., Nagarajan, V.K., Lahner, B., Meaghe,r R.B., Raghothama, K.G.: Variations in the composition of gelling agents affect morphophysiological and molecular responses to deficiencies of phosphate and other nutrients. — Plant Physiol. 150: 1033–1049, 2009.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Kisko, M., Bouain, N., Rouached, A., Choudhary, S.P., Rouached, H.: Molecular mechanisms of phosphate and zinc signalling crosstalk in plants: phosphate and zinc loading into root xylem in Arabidopsis. — Environ. exp. Bot. 114: 57–64, 2015.

    Article  CAS  Google Scholar 

  • Lambers, H., Hayes, P.E., Laliberté, E., Oliveira, R.S., Turner, B.L.: Leaf manganese accumulation and phosphorusacquisition efficiency. — Trends Plant Sci. 20: 83–90, 2015.

    Article  CAS  PubMed  Google Scholar 

  • Lambers, H., Shane, M.W., Cramer, M.D., Pearse, S.J., Veneklaas, E.J.: Root structure and functioning for efficient acquisition of phosphorus: matching morphological and physiological traits. — Ann. Bot. 98: 693–713, 2006.

    Article  PubMed  PubMed Central  Google Scholar 

  • Müller, J., Toev, T., Heisters, M., Teller, J., Moore, K.L., Hause, G., Dinesh, D.C., Bürstenbinder, K., Abel, S.: Irondependent callose deposition adjusts root meristem maintenance to phosphate availability. — Dev. Cell 33: 216–230, 2015.

    Article  PubMed  Google Scholar 

  • Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures. — Physiol. Plant. 15: 473–479, 1962.

    Article  CAS  Google Scholar 

  • Ogawa, S., Valencia, M., Ishitani, M., Selvaraj, M.: Root system architecture variation in response to different NH4 + concentrations and its association with nitrogen-deficient tolerance traits in rice. — Acta Physiol Plant 36: 2361–2372, 2014.

    Article  CAS  Google Scholar 

  • Péret, B., Desnos, T., Jost, R., Kanno, S., Berkowitz, O., Nussaume, L.: Root architecture responses: in search of phosphate. — Plant Physiol. 166: 1713–1723, 2014.

    Article  PubMed  PubMed Central  Google Scholar 

  • Somerville, C.R., Ogren, W.: Isolation of photorespiration mutants in Arabidopsis thaliana. -In: Edelman, M. (ed.): Methods in Chloroplast Biology. Pp. 129–138. Elsevier Biomedical Press, Amsterdam 1982.

    Google Scholar 

  • Stefanovic, A. Ribot, C., Rouached, H., Wang, Y., Chong, J., Belbahri, L., Delessert, S., Poirie, Y: Members of the pho1 gene family show limited functional redundancy in phosphate transfer to the shoot, and are regulated by phosphate deficiency via distinct pathways. - Plant J. 50: 982–994, 2007.

    Article  CAS  PubMed  Google Scholar 

  • Svistoonoff, S., Creff, A., Reymond, M., Sigoillot-Claude, C., Ricaud, L., Blanchet, A., Nussaume, L., Desnos, T.: Root tip contact with low-phosphate media reprograms plant root architecture. — Nat. Genet. 39: 792–796, 2007.

    Article  CAS  PubMed  Google Scholar 

  • Vitha, S., Benes, K., Phillips, J.P., Gartland, K.M.A.: Histochemical GUS analysis. - In: Gartland, K.M.A., Davey, M.R. (ed.): Agrobacterium Protocols. Pp. 185–193. Humana Press, Totowa 1995.

    Chapter  Google Scholar 

  • Ward, J.T., Lahne,r B., Yakubova, E., Salt, D.E., Raghothama, K.G.: The effect of iron on the primary root elongation of Arabidopsis during phosphate deficiency. — Plant Physiol. 147: 1181–1191, 2008.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Zheng, L., Huang, F., Narsai, F., Wu, J., Giraud, E., He, F., Cheng, L., Wang, F., Wu, P., Whelan, J., Shou, H.: Physiological and transcriptome analysis of iron and phosphorus interaction in rice seedlings. — Plant Physiol. 151: 262–274, 2009.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

Author information

Author notes

  1. M. L. Strieder

    Present address: Embrapa Wheat, 99001-970, Passo Fundo, RS, Brazil

Authors and Affiliations

  1. Department of Crop Science, Federal University of Rio Grande do Sul, 91501-970, Porto Alegre, RS, Brazil

    M. L. Strieder, K. G. Pinto, C. Bertoldi, A. de B. Schneider & C. A. Delatorre

  2. Institute of Developmental Genetics, Heinrich Heine University, D-40225, Düsseldorf, Germany

    M. L. Strieder & K. G. Pinto

  3. Department of Bioinformatics and Genomics, University of North Carolina, Charlotte, NC, 9201, USA

    A. de B. Schneider

Authors
  1. M. L. Strieder
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. K. G. Pinto
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. C. Bertoldi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A. de B. Schneider
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. C. A. Delatorre
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to C. A. Delatorre.

Additional information

Acknowledgments: This work was supported by the Brazilian National Council for Scientific and Technological Development (CNPq) Grants No. 473050/2006-4 and 470173/2010-6. MLS received a pre-doctoral fellowship (Proc. 140725/2006-7 and 201660/2007-5) and CAD received PQ-1D fellowship (Proc. 305122/2014-2) from CNPq.

Electronic supplementary material

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Strieder, M.L., Pinto, K.G., Bertoldi, C. et al. Response of Arabidopsis thaliana root growth to phosphorus and its relation to media chemical composition. Biol Plant 61, 587–594 (2017). https://doi.org/10.1007/s10535-017-0713-z

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10535-017-0713-z

Additional key words

Search

Navigation