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Altered gibberellin content affects growth and development in transgenic tobacco lines overexpressing a wheat gene encoding F-box protein

  • Original Paper
  • Published:
Biologia Plantarum

Abstract

In a previous study, we have identified and characterized gene from wheat (Triticum aestivum L.) encoding F-box protein and named it TaFBA. In this paper, transgenic tobacco (Nicotiana tabacum L.) plants overexpressing TaFBA1 displayed accelerated growth early, but the rate slowed gradually at later stages of growth, and the mature transgenic plants were even shorter in stature and flowered later than did the wild type (WT). Treatment with gibberellin (GA) conferred an accelerated growth rate to the transgenic tobacco plants at later stages, similar to that of WT, whereas growth was inhibited more seriously in WT than in transgenic tobacco when plants were treated with a GA biosynthesis inhibitor. The content of GA in transgenic tobacco plants was higher at early developmental stages, but it was lower at later growth stages than in WT. Some GA biosynthesis genes were down regulated, which was accompanied with elevated expression of a GA catabolism gene. Thus, our results suggest that TaFBA1 is possibly involved in the regulation of plant growth and development, and that it may be related to the production, metabolism, and proper function of GA.

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Abbreviations

ELISA:

enzyme-linked immunosorbent assay

GA:

gibberellin

PAC:

paclobutrazol

UPS:

ubiquitin-26S proteasome system

WT:

wild-type

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Authors and Affiliations

  1. State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Life Science, Shandong Agricultural University, Tai’an, Shandong, 271018, P.R. China

    S. Yin, S. Zhou, X. Kong, Y. Han & W. Wang

Authors
  1. S. Yin
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  2. S. Zhou
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  3. X. Kong
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  4. Y. Han
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  5. W. Wang
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Correspondence to W. Wang.

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Acknowledgements: This study was supported by the National Natural Science Foundation of China (No. 31370304), by the Natural Science Foundation of Shandong Province, China (No. ZR2015CL037), and by the Opening Foundation of State Key Laboratory of Crop Biology (2013KF01). The first two authors contributed equally to this paper.

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Yin, S., Zhou, S., Kong, X. et al. Altered gibberellin content affects growth and development in transgenic tobacco lines overexpressing a wheat gene encoding F-box protein. Biol Plant 61, 349–358 (2017). https://doi.org/10.1007/s10535-017-0707-x

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  • DOI: https://doi.org/10.1007/s10535-017-0707-x

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