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Alleviation of salt-induced oxidative stress in rice seedlings by proline and/or glycinebetaine

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Biologia Plantarum

Abstract

The effects of proline and/or glycine betaine (GB) application on growth, photosynthetic pigments, H2O2 content, and activities of antioxidant enzymes in rice (Oryza sativa L. cv. KDML105) under salt stress were investigated. The H2O2 content and the activities of superoxide dismutase (SOD), glutathione reductase (GR), and ascorbate peroxidase (APX) but not catalase (CAT) increased under salinity. Under 160 mM NaCl, the CAT activity was maintained on the pre-stress level in the presence of proline, whereas in the presence of GB, the GR activity increased more than without GB application. A co-application of 30 mM proline and 1 mM GB did not reduce the increase in H2O2 caused by the NaCl stress more than applying each of the osmoprotectants and no synergistic effect on the antioxidant enzymes was observed. However, the application of both the osmoprotectants was the most effective in alleviating degradation of photosynthetic pigments.

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Abbreviations

APX:

ascorbate peroxidase (EC 1.11.1.11)

CAT:

catalase (EC 1.11.1.6)

GB:

glycinebetaine

GR:

glutathione reductase (EC 1.6.4.2)

GSSG:

oxidized glutathione

H2O2 :

hydrogen peroxide

ROS:

reactive oxygen species

SOD:

superoxide dismutase (EC 1.15.1.1)

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Science, Chulalongkorn University, 254 Phayathai Road, Patumwan, Bangkok, 10330, Thailand

    N. Wutipraditkul, P. Wongwean & T. Buaboocha

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  1. N. Wutipraditkul
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  2. P. Wongwean
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  3. T. Buaboocha
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Correspondence to N. Wutipraditkul.

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Acknowledgments: This work was supported by the research group “Special Task Force for Activating Research (STAR): Biochemical and Molecular Mechanisms of Rice in Changing Environments” by the Ratchadaphiseksompot Endowment Fund, Chulalongkorn University and Thailand Research Fund IRG578008. We thank Dr. Supa-art Sirikantaramas for the valuable guidance and Miss Taviporn Kaewneramit for support.

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Wutipraditkul, N., Wongwean, P. & Buaboocha, T. Alleviation of salt-induced oxidative stress in rice seedlings by proline and/or glycinebetaine. Biol Plant 59, 547–553 (2015). https://doi.org/10.1007/s10535-015-0523-0

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  • DOI: https://doi.org/10.1007/s10535-015-0523-0

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