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Accumulation of WCS120 and DHN5 proteins in differently frost-tolerant wheat and barley cultivars grown under a broad temperature scale

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Biologia Plantarum

Abstract

Proteins WCS120 and DHN5 are known as the major cold-inducible dehydrins in wheat and barley plants, respectively. WCS120 and DHN5 relative accumulation increased exponentially along with a growth temperature decline in the range from optimum to cold temperatures. Even at optimum growth temperatures, the most frost-tolerant wheat and barley cultivars can be distinguished from the remaining ones according to dehydrin relative accumulation. The highly tolerant wheat and barley cultivars started accumulating dehydrins at higher growth temperatures and reached higher dehydrin amounts than the less tolerant ones. Statistically significant correlations between lethal temperature for 50 % of the samples (LT50) and dehydrin relative accumulation have been found at all growth temperatures (5, 10, 15 and 20 °C) for WCS120 in wheats and at 5 and 10 °C for DHN5 in barleys. Analogous relationships between dehydrin relative accumulation at different growth temperatures and plant acquired frost tolerance have been proved for wheat WCS120 and barley DHN5.

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Abbreviations

ALP:

alkaline phosphatase

CA:

cold acclimation

CBF:

C-repeat binding factor

COR:

cold-regulated

DD:

degree days (days of cultivation multiplied by growth temperature)

DHN:

dehydrin

FR:

frost-resistance

FT:

frost tolerance

GAR:

goat anti-rabbit (secondary antibody)

LEA:

late embryogenesis abundant

LT50:

lethal temperature when 50 % of sample die

PAGE:

polyacrylamide gel electro-phoresis

SDS:

sodium dodecyl sulfate

TBS:

Tris-buffered saline

TTBS:

Tween-20 Tris-buffered saline

WCS:

wheat cold-specific

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Authors and Affiliations

  1. Department of Genetics and Plant Breeding, Crop Research Institute, Drnovská 507, CZ-16106, Prague 6 - Ruzyně, Czech Republic

    K. Kosová, P. Vítámvás, P. Prášilová & I. T. Prášil

Authors
  1. K. Kosová
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  2. P. Vítámvás
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  3. P. Prášilová
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  4. I. T. Prášil
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Correspondence to K. Kosová.

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Acknowledgements: The work was supported by two postdoctoral projects of the Grant Agency of the Czech Republic GA CR P501/11/P637 and GP522/09/P621, National Agency for Agricultural Research MZe QH91158 and Ministry of Agriculture of the Czech Republic MZe 0002700604.

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Kosová, K., Vítámvás, P., Prášilová, P. et al. Accumulation of WCS120 and DHN5 proteins in differently frost-tolerant wheat and barley cultivars grown under a broad temperature scale. Biol Plant 57, 105–112 (2013). https://doi.org/10.1007/s10535-012-0237-5

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  • DOI: https://doi.org/10.1007/s10535-012-0237-5

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