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Ex vitro acclimatization of plantain plantlets micropropagated in temporary immersion bioreactor

  • Original Papers
  • Published:
Biologia Plantarum

Abstract

Plantain (Musa ABB CEMSA 3/4) plantlets were micropropagated in temporary immersion bioreactors (TIB) or in gelled medium (GM). After ex vitro transfer ROS accumulation was determined by infiltrating leaves with nitroblue tetrazolium (NBT) and 3,3′-diaminobenzidine (DAB). Stomatal cells were more stained with NBT and DAB in GM plants than in TIB plants, but the difference disappeared at the end of acclimatization. At the end of the in vitro phase, GM plantlets showed higher activities of ascorbate peroxidase (APX), dehydroascorbate reductase (DHAR), monodehydroascorbate reductase (MDHAR) and glutathione reductase (GR), while activities of catalase (CAT), superoxide dismutase (SOD) and glutathione transferase (GT) were higher in TIB grown plantlets. At the end of acclimatization GT, SOD, CAT and MDHAR stabilized at low values of activity in plantlets derived from both treatments. Concerning the correspondent genes, GM plantlets showed higher expression of all transcripts with the exception of CuZnSOD. The immunobloting of peroxiredoxins (PRXs) showed that chloroplast-located PRXs were expressed at higher levels in TIB plantlets, some showing polymerization. In conclusion, TIB grown plantlets had an improved anti-oxidative response when compared with GM.

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Abbreviations

APX:

ascorbate peroxidase (EC 1.11.1.11)

BA:

6-benzylaminopurine

CAT:

catalase (EC 1.11.1.6)

DAB:

3,3′-diaminobenzidine

DHAR:

dehydroascorbate reductase (EC 1.8.5.1)

GM:

gelled medium

GR:

glutathione reductase (EC 1.6.4.2)

GT:

glutathione transferase (EC 2.5.1.18)

MDHAR:

monodehydroascorbate reductase (EC 1.6.5.4)

MS:

Murashige and Skoog medium

NBT:

nitroblue tetrazolium

PPFD:

photosynthetic photon flux density

PRX:

peroxiredoxin

ROS:

reactive oxygen species

SOD:

superoxide dismutase (EC 1.15.1.1)

TIB:

temporary immersion bioreactor

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Acknowledgements

To the program Alban, scholarship number E06D100217CU (C.A), to “Fundação para a Ciência e Tecnologia”, co-financed by FEDER for post-doc grant SFRH/BPD/5707/2001 (L.C.) and “Plurianual” funds to CBAA.

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Authors and Affiliations

  1. Departamento de Botânica e Engenharia Biologica, Instituto Superior de Agronomia, Universidade Técnica de Lisboa, 1349-017, Lisboa, Portugal

    C. Aragón, L. Carvalho & S. Amâncio

  2. Laboratorio de Células y Cultivo Tejidos, Centro de Bioplantas, Universidad de Ciego de Ávila, Ciego de Ávila, Cuba

    C. Aragón, J. González & M. Escalona

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  1. C. Aragón
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  2. L. Carvalho
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  3. J. González
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  4. M. Escalona
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  5. S. Amâncio
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Correspondence to S. Amâncio.

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Aragón, C., Carvalho, L., González, J. et al. Ex vitro acclimatization of plantain plantlets micropropagated in temporary immersion bioreactor. Biol Plant 54, 237–244 (2010). https://doi.org/10.1007/s10535-010-0042-y

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  • DOI: https://doi.org/10.1007/s10535-010-0042-y

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