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Changes in key enzymes of viral-RNA biosynthesis in chloroplasts from PVY and TMV infected tobacco plants

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  • Published:
Biologia Plantarum

Abstract

Effects of the infection with tobacco mosaic virus (TMV) and potato virus Y (PVY) on chloroplasts from susceptible tobacco plants were determined. Changes in ribonucleases (RNases), phosphomonoesterase (PME), phosphodiesterase (PDE), glucose-6-phosphate dehydrogenase (G6P DH), 6-phosphogluconate dehydrogenase (6PG DH), glucokinase (GK), and fructokinase (FK) activities in thylakoid/envelope and stroma fractions were studied. Slight increase in the activities of PME, PDE, G6P DH and 6PG DH of thylakoid/envelope fraction as well as of RNases, PME, PDE, G6P DH, 6PG DH, GK and FK of stroma fraction was found in chloroplasts isolated from leaf tissues infected with PVY. Infection with TMV produced higher increase in enzymes activities in chloroplasts; especially, PME, G6P DH and 6PG DH in fraction of thylakoid/envelope, and RNases, PME, PDE, G6P DH, 6PG DH, and GK in stroma fraction.

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Abbreviations

dpi:

day post virus inoculation

FK:

fructokinase

GK:

glucokinase

G6P DH:

glucose-6-phosphate dehydrogenase

PDE:

phosphodiesterase

6PG:

DH 6-phosphogluconate dehydrogenase

PME:

phosphomonoesterase

PVY:

potato virus Y

RNases:

ribonucleases

TMV:

tobacco mosaic virus

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Authors and Affiliations

  1. Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Na Karlovce 1, CZ-16000, Prague 6, Czech Republic

    M. Sindelarova, L. Sindelar, N. Wilhelmova & D. Prochazkova

Authors
  1. M. Sindelarova
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  2. L. Sindelar
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  3. N. Wilhelmova
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  4. D. Prochazkova
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Corresponding author

Correspondence to M. Sindelarova.

Additional information

This study was supported by grant No. 522/02/0708 of the Grant Agency of the Czech Republic.

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Sindelarova, M., Sindelar, L., Wilhelmova, N. et al. Changes in key enzymes of viral-RNA biosynthesis in chloroplasts from PVY and TMV infected tobacco plants. Biol Plant 49, 471–474 (2005). https://doi.org/10.1007/s10535-005-0032-7

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  • DOI: https://doi.org/10.1007/s10535-005-0032-7

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