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Geographic and genetic variation in susceptibility of Butomus umbellatus to foliar fungal pathogens

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Abstract

Large-scale patterns of plant invasions may reflect regional heterogeneity in biotic and abiotic factors and genetic variation within and between invading populations. Having information on how effects of biotic resistance vary spatially can be especially important when implementing biological control because introduced agents may have different impacts through interactions with host-plant genotype, local environment, or other novel enemies. We conducted a series of field surveys and laboratory studies to determine whether there was evidence of biotic resistance, as foliar fungal pathogens, in two introduced genotypes (triploid G1, diploid G4) of the Eurasian wetland weed, Butomus umbellatus L. in the USA. We tested whether genotypes differed in disease attack and whether spatial patterns in disease incidence were related to geographic location or climate for either genotype. We surveyed 27 B. umbellatus populations (17 G1, 10 G4) to determine disease incidence and associated fungal pathogens. For a subset of plant populations, we isolated foliar fungi and tested pathogenicity of three isolates in laboratory assays. After accounting for location (latitude, climate), G1 plants had lower disease incidence than G4 plants in the field (38% vs. 70%) but similar pathogen richness. In contrast, bioassays revealed G1 plants consistently received a higher damage score and had larger leaf lesions regardless of pathogen. The seemingly contradictory results between the field and laboratory may be due to climatic differences between areas that limit the regional pool of pathogens or their effect on plant genotype. These results demonstrate that two widespread B. umbellatus genotypes exhibit different susceptibility to pathogens and effectiveness of pathogen biological controls may depend on local conditions.

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Acknowledgements

The authors thank the following people for their assistance in the field, laboratory, or greenhouse to complete this work: J. Parsons, C. Welling, B. DeRossette, and D. Lattuca. Additionally, we thank Aaron Schad and Lynde Dodd for their reviews of this manuscript. Their suggestions, along with those from two anonymous reviewers greatly improved the quality of the manuscript. This work was conducted in partial fulfillment of the requirements for the degree of Doctor of Philosophy at Louisiana State University (NEH).

Funding

This research was supported by the US Army Engineer Research and Development Center Aquatic Plant Control Research Program.

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  1. U.S. Army Engineer Research and Development Center, 3909 Halls Ferry Rd., Vicksburg, MS, 39180, USA

    Nathan Harms & Judy Shearer

  2. Department of Biological Sciences, Louisiana State University, 202 Life Sciences Building, Baton Rouge, LA, 70803, USA

    Nathan Harms & James T. Cronin

  3. U.S. Department of Agriculture, Agricultural Research Service, 1500 N. Central Ave., Sidney, MT, 59270, USA

    John F. Gaskin

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Harms, N., Shearer, J., Cronin, J.T. et al. Geographic and genetic variation in susceptibility of Butomus umbellatus to foliar fungal pathogens. Biol Invasions 22, 535–548 (2020). https://doi.org/10.1007/s10530-019-02109-3

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