Abstract
The comparative transcriptome analysis of the fungus Gibberella zeae which could efficiently catalyze the 7β-hydroxylation of LCA to produce UDCA was performed with LCA induction. This is the first time to report the comparative transcriptome of fungus under LCA treatment. Totally, 1364 differentially expressed genes including 770 up-regulated and 594 down-regulated genes were identified. In the 770 up-regulated genes, 12 genes with the function of hydroxylation were picked out by application of function screening, which were annotated as CYP450 or hydroxylase. Moreover, the qRT-PCR results of five up-regulated CYP450-like genes confirmed the credibility of RNA-Seq further. These results provide valuable information for the discovery of novel enzyme producing clinical drug UDCA from butchery byproduct LCA, and also might indicate some clues for the detoxification process of LCA in humans.
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Acknowledgements
This work was supported by the National Natural Science Foundation of China (No. 21807009); the National Science and Technology Major Projects for “Major New Drugs Innovation and Development” (No. 2017ZX09309006-003); and the Fundamental Research Funds for the Central Universities of China (Nos. 106112017CDJXY230003 and No. 2019CDXYSG0004).
Supporting information
Cultivation and bioconversion conditions. The identification of the product UDCA. Supplementary Fig. S1—TLC detection for the bioconversion of LCA into UDCA with G. zeae. Supplementary Fig. S2—HPLC spectra of the bioconversion of LCA into UDCA with G. zeae. Supplementary Fig. S3—The length distribution of the transcripts of G. zeae VKM F-2600. Supplementary Fig. S4—The GO analysis of all the genes of G. zeae VKM F-2600. Supplementary Fig. S5—The KEGG analysis of all the genes of G. zeae VKM F-2600. Supplementary Table S1—Primers used for qRT-PCR analysis. Supplementary Table S2—Sequencing quality of control and LCA-induced groups. Supplementary Table S3—Read mapping results of the sequences in the control and LCA-induced groups. Supplementary Table S4—Functional annotation of transcriptome data.
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Yang, B., Zha, R., Zhao, W. et al. Comparative transcriptome analysis of the fungus Gibberella zeae transforming lithocholic acid into ursodeoxycholic acid. Biotechnol Lett 43, 415–422 (2021). https://doi.org/10.1007/s10529-020-03048-z
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DOI: https://doi.org/10.1007/s10529-020-03048-z