Abstract
Objective
To compare methods for erythroid differentiation of K562 cells that will be promising in the treatment of beta-thalassemia by inducing γ-globin synthesis.
Results
Cells were treated separately with: RPMI 1640 medium without glutamine, RPMI 1640 medium without glutamine supplemented with 1 mM sodium butyrate, RPMI 1640 medium supplemented with 1 mM sodium butyrate, 25 µg cisplatin/ml, 0.1 µg cytosine arabinoside/ml. The highest differentiation (84 %) with minimum toxicity was obtained with cisplatin at 15 µg /ml. Real-time RT-PCR showed that expression of the γ-globin gene was significantly higher in the cells differentiated with cisplatin compared to undifferentiated cells (P < 0.001).
Conclusions
Cisplatin is useful in the experimental therapy of ß-globin gene defects and can be considered for examining the basic mechanism of γ-reactivation.
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Acknowledgments
We are deeply grateful to Tehran University of Medical Sciences deputy of research (Grant No. 92-03-87-21886) and Iran national science foundation (Grant No. 92005949) due to their support.
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Shariati, L., Modaress, M., Khanahmad, H. et al. Comparison of different methods for erythroid differentiation in the K562 cell line. Biotechnol Lett 38, 1243–1250 (2016). https://doi.org/10.1007/s10529-016-2101-8
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DOI: https://doi.org/10.1007/s10529-016-2101-8